Anticorps Polyclonal de lapin anti-STAG1

STAG1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 14015-1-AP

Synonymes

Cohesin subunit SA 1, SA1, SCC3 homolog 1, STAG1, stromal antigen 1

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Galerie de données de validation

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  WB analysis of human heart using 14015-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 14015-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 14015-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14015-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules Raji, tissu cardiaque humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

14015-1-AP cible STAG1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STAG1 Protéine recombinante Ag5123
• Nom complet: stromal antigen 1
• Masse moléculaire calculée: 144 kDa
• Poids moléculaire observé: 155 kDa
• Numéro d’acquisition GenBank: BC064699
• Symbole du gène: STAG1
• Identification du gène (NCBI): 10274
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAG1, also named as SCC3 homolog 1, is a 1258 amino acid protein, which belongs to the SCC3 family. STAG1 interacts directly with RAD21 in cohesin complex and localizes in nucleus. STAG1 is a Component of cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The calculate molecular weight of STAG1 is 144 kDa, but modified STAG1 is about 145-150 kDa.

Publications

Species	Application	Title
rat	WB	Bone Res; Circular RNA circStag1 promotes bone regeneration by interacting with HuR.; Authors - Gaoyang Chen
human	WB	Proc Natl Acad Sci U S A; Genetic analysis of cancer drivers reveals cohesin and CTCF as suppressors of PD-L1.; Authors - Ena Oreskovic