Anticorps Polyclonal de lapin anti-STAG2

STAG2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 19837-1-AP

Synonymes

Cohesin subunit SA 2, SA 2, SA2, SCC3 homolog 2, STAG2, stromal antigen 2

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Galerie de données de validation

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  WB analysis of Jurkat using 19837-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 19837-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 19837-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19837-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 19837-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 19837-1-AP

  IP result of anti-STAG2(IP:19837-1-AP, 4ug; Detection:19837-1-AP 1:3000) with MCF-7 cells lysate 2120 ug.

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  IP experiment of U-251 using 19837-1-AP

  IP result of anti-STAG2(IP:19837-1-AP, 4ug; Detection:19837-1-AP 1:3000) with U-251 cells lysate 3320 ug.

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  IHC staining of human cervical cancer using 19837-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19837-1-AP (STAG2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 19837-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19837-1-AP (STAG2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HEK-293 using 19837-1-AP

  Immunofluorescent analysis of 293 cells, using STAG2 antibody 19837-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules K-562, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IP: cellules U-251, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

19837-1-AP cible STAG2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STAG2 Protéine recombinante Ag13825
• Nom complet: stromal antigen 2
• Masse moléculaire calculée: 1231 aa, 141 kDa
• Poids moléculaire observé: 130-141 kDa
• Numéro d’acquisition GenBank: BC001765
• Symbole du gène: STAG2
• Identification du gène (NCBI): 10735
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAG2, also named as SA2, is a component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex may play a role in spindle pole assembly during mitosis. Mutations in STAG2 appear to be a first step in the transformation of a normal cell into a cancer cell.

Publications

Species	Application	Title
human	WB	Cancer Commun (Lond); Squalene epoxidase promotes colorectal cancer cell proliferation through accumulating calcitriol and activating CYP24A1-mediated MAPK signaling.; Authors - Luwei He