Anticorps Polyclonal de lapin anti-STAMBP

• Phare
• Validé par KD/KO

STAMBP Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 11346-1-AP

Synonymes

AMSH, EC:3.4.19.-, Endosome-associated ubiquitin isopeptidase, STAM binding protein, STAM-binding protein

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Galerie de données de validation

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  WB analysis using 11346-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11346-1-AP (STAMBP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11346-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11346-1-AP (STAMBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11346-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11346-1-AP (STAMBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 11346-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11346-1-AP (STAMBP antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562, tissu cérébral de souris, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

11346-1-AP cible STAMBP dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STAMBP Protéine recombinante Ag1895
• Nom complet: STAM binding protein
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC007682
• Symbole du gène: STAMBP
• Identification du gène (NCBI): 10617
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAM-binding protein (STAMBP) is also named AMSH. STAMBP, a member of the Jab1/MPN metalloenzyme family of deubiquitinating enzymes (DUBs), specifically cleaves K63-linked polyubiquitination chains from substrates (PMID: 15314065, PMID: 26601948). STAMBP is implicated in the endosome-lysosome pathway and shuttles ubiquitinated substrate proteins to lysosomes for degradation (PMID: 10383417, PMID: 24151880). STAMBP regulates protein degradation by binding to the ESCRT-0 protein STAM and the charged multivesicular body proteins of the ESCRT-III complex (PMID: 20159979). STAMBP is implicated in the inflammatory response by promoting the deubiquitination and stabilization of NALP7 and increasing the activity of inflammasomes (PMID: 28492230). STAMBP expression was increased in the cytoplasm of tumor cells from LUAD patients. STAMBP overexpression promoted cell migration and invasion, whereas STAMBP knockdown attenuated these processes in LUAD cells after epidermal growth factor treatment (PMID: 3410245).

Publications

Species	Application	Title
human	WB	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
human	WB,IHC,IF,CoIP	Cell Death Dis; Identification of STAM-binding protein as a target for the treatment of gemcitabine resistance pancreatic cancer in a nutrient-poor microenvironment; Authors - Wenming Zhang ; KD Validated
human	WB	J Biol Chem; Endosomal deubiquitinating enzymes control ubiquitination and down-regulation of protease-activated receptor 2.; Authors - Hasdemir Burcu B
human	WB	Mol Biol Cell; Arrestin-2 interacts with the endosomal sorting complex required for transport machinery to modulate endosomal sorting of CXCR4.; Authors - Malik Rohit R ; KD Validated

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