Anticorps Recombinant de lapin anti-STAT1

• Phare
• Validé par KD/KO

STAT1 Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

Publications(2)

CloneNo.

2F16

N° de cat : 82016-1-RR

Synonymes

2F16, ISGF 3, Signal transducer and activator of transcription 1-alpha/beta, STAT91, Transcription factor ISGF-3 components p91/p84

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Galerie de données de validation

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  WB analysis using 82016-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82016-1-RR (STAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 82016-1-RR

  WB result of STAT1 antibody (82016-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT1 transfected HEK-293 cells.

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  IP experiment of PC-3 using 82016-1-RR

  IP result of anti-STAT1 (IP:82016-1-RR, 4ug; Detection:82016-1-RR 1:10000) with PC-3 cells lysate 2000 ug.

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  IHC staining of human ovary tumor using 82016-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 82016-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 82016-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 82016-1-RR

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 82016-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 82016-1-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 82016-1-RR

  Immunofluorescent analysis of (4% PFA) fixed IFN alpha treated HeLa cells using STAT1 antibody (82016-1-RR, Clone: 2F16 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  ChIP experiment of HT-1080 using 82016-1-RR

  Chromatin was prepared from HT-1080 cells either non-treated or treated with hIFN-γ (50 ng/ml, 30min). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of STAT1 (82016-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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  Affinity and Kinetic Characterization of 82016-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82016-1-RR against Human STAT1 were performed. The affinity constant is 0.501 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3
• Résultats positifs en IP: cellules PC-3,
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de côlon de rat, tissu de côlon de souris, tissu splénique de rat, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: IFN alpha treated HeLa cells,
• Résultats positifs en ChIP-qPCR: IFN-γ -HT-1080 (50 ng/ml, 30min) HT-1080 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

82016-1-RR cible STAT1 dans les applications de WB, IHC, IF/ICC, IP, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: STAT1 Protéine recombinante Ag0199
• Nom complet: signal transducer and activator of transcription 1, 91kDa
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC002704
• Symbole du gène: STAT1
• Identification du gène (NCBI): 6772
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. Various ligands, including interferons- and - , EGF, PDGF and IL-6, can activate STAT1. STAT1 protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.

Publications

Species	Application	Title
human	WB	Noncoding RNA Res; LncRNA RNF144A-AS1 gene polymorphisms and their influence on lung cancer patients in the Chinese Han population; Authors - Hongjiao Wu
human	WB	Cell Biochem Biophys; FOXA1 Transcriptional Repression of PLSCR1 Inhibits Tongue Squamous Cell Carcinoma Progression; Authors - Jinxin Wang

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