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  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-STAT3

STAT3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(569)

N° de cat : 10253-2-AP

Synonymes

Acute-phase response factor, APRF, HIES, STAT 3



Applications testées

Résultats positifs en WBcellules A549, cellules A431, cellules HeLa, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3, cellules PC-12
Résultats positifs en IPcellules HeLa,
Résultats positifs en IHCtissu de cancer de l'estomac humain, tissu de cancer du col de l'utérus humain, tissu de cancer du côlon humain, tissu de cancer du sein humain, tissu de côlon de souris
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HeLa, IFN alpha treated HeLa cells
Résultats positifs en FC (Intra)cellules Ramos,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

10253-2-AP cible STAT3 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, bovin, Humain, poisson-zèbre, porc, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène STAT3 Protéine recombinante Ag0360
Nom complet signal transducer and activator of transcription 3 (acute-phase response factor)
Masse moléculaire calculée 770 aa, 88 kDa
Poids moléculaire observé 88 kDa
Numéro d’acquisition GenBankBC000627
Symbole du gène STAT3
Identification du gène (NCBI) 6774
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.

Protocole

Product Specific Protocols
WB protocol for STAT3 antibody 10253-2-APDownload protocol
IHC protocol for STAT3 antibody 10253-2-APDownload protocol
IF protocol for STAT3 antibody 10253-2-APDownload protocol
IP protocol for STAT3 antibody 10253-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell

Targeting the immune privilege of tumor-initiating cells to enhance cancer immunotherapy

Authors - Chen Yang
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
mouse,humanWB

Cell Metab

Lactate modulates iron metabolism by binding soluble adenylyl cyclase

Authors - Wei Liu
mouseWB

Cell Mol Immunol

Malonate promotes CD8+ T cell memory formation via protein malonylation

Authors - Qianqian Duan
mouseWB

Brain Behav Immun

l-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation.

Authors - Danqing Xin
mouseWB

EMBO Mol Med

Targeting HPK1 inhibits neutrophil responses to mitigate post-stroke lung and cerebral injuries

Authors - Tingting Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Iram (Verified Customer) (09-18-2025)

Bands are very clear band.

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: CRC cells
FH

Daniele (Verified Customer) (04-29-2022)

It works very well for WB, IF and IP

  • Applications: Western Blot, Immunofluorescence, Other
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: TNBC
FH

Yuan (Verified Customer) (01-23-2020)

Very good Stat3 antibody for western blot. Strong signal at 1:5000 dilution. Highly recommend.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Hela
FH

Daniele (Verified Customer) (11-25-2019)

It works very well both in western blot and in immunoprecipitation.

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000 (western blot)
  • Cell Tissue Type: MDA MB 468 and A375
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