Anticorps Polyclonal de lapin anti-STAT3

• Phare
• Validé par KD/KO

STAT3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

373

Avis (3)

N° de cat : 10253-2-AP

Synonymes

Acute phase response factor, APRF, HIES, STAT3

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Galerie de données de validation

• 

  WB analysis using 10253-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10253-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10253-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10253-2-AP

  WB result of STAT3 antibody (10253-2-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT3 transfected A549 cells.

• 

  WB analysis of rat brain using 10253-2-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 10253-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10253-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10253-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10253-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10253-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10253-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10253-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10253-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10253-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10253-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10253-2-AP

  IP Result of anti-STAT3 (IP:10253-2-AP, 3ug; Detection:10253-2-AP 1:2000) with HeLa cells lysate 3000ug.

• 

  IHC staining of human stomach cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 10253-2-AP (STAT3 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 10253-2-AP (STAT3 Antibody) at dilution of 1:50.

• 

  IHC staining of mouse colon using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10253-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STAT3 antibody (10253-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). DAPI (blue).

• 

  IF Staining of HeLa using 10253-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STAT3 antibody (10253-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 10253-2-AP

  Immunofluorescent analysis of HeLa cells using 10253-2-AP (STAT3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of Ramos using 10253-2-AP

  1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human STAT3 (10253-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A431, cellules HeLa, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3, cellules PC-12, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral de souris, tissu pulmonaire de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer du col de l'utérus humain, tissu de cancer du côlon humain, tissu de cancer du sein humain, tissu de côlon de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules Ramos,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 22 publications below
• WB: See 342 publications below
• IHC: See 27 publications below
• IF: See 28 publications below
• IP: See 7 publications below
• CoIP: See 5 publications below
• ChIP: See 10 publications below

Informations sur le produit

10253-2-AP cible STAT3 dans les applications de WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STAT3 Protéine recombinante Ag0360
• Nom complet: signal transducer and activator of transcription 3 (acute-phase response factor)
• Masse moléculaire calculée: 770 aa, 88 kDa
• Poids moléculaire observé: 88 kDa
• Numéro d’acquisition GenBank: BC000627
• Symbole du gène: STAT3
• Identification du gène (NCBI): 6774
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human,mouse	WB	Cell Metab; Lactate modulates iron metabolism by binding soluble adenylyl cyclase; Authors - Wei Liu
mouse	WB	Brain Behav Immun; l-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation.; Authors - Danqing Xin
mouse	WB	ACS Appl Mater Interfaces; A Versatile Nanoplatform for Broad-Spectrum Immunotherapy by Reversing the Tumor Microenvironment; Authors - Ao Chen
mouse	WB	J Nanobiotechnology; Nanocomposites based on nanoceria regulate the immune microenvironment for the treatment of polycystic ovary syndrome; Authors - Sisi Yan
human	WB,ChIP	Redox Biol; Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer.; Authors - Shumin Ouyang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniele (Verified Customer) (04-29-2022)

It works very well for WB, IF and IP

• Applications: Western Blot, Immunofluorescence, Other
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: TNBC

FH

Yuan (Verified Customer) (01-23-2020)

Very good Stat3 antibody for western blot. Strong signal at 1:5000 dilution. Highly recommend.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Hela

FH

Daniele (Verified Customer) (11-25-2019)

It works very well both in western blot and in immunoprecipitation.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000 (western blot)
• Cell Tissue Type: MDA MB 468 and A375

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