• Phare
  • Validé par KD/KO

Anticorps Monoclonal anti-STAT3

STAT3 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

CloneNo.

3G2D12

N° de cat : CL555-60199

Synonymes

Acute phase response factor, APRF, FLJ20882, HIES, STAT3



Applications testées

Résultats positifs en cytométriecellules HepG2

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-60199 cible STAT3 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Mouse / IgG2a
Clonalité Monoclonal
Type Anticorps
Immunogène STAT3 Protéine recombinante Ag0360
Nom complet signal transducer and activator of transcription 3 (acute-phase response factor)
Masse moléculaire calculée 770 aa, 88 kDa
Poids moléculaire observé 88 kDa
Numéro d’acquisition GenBankBC000627
Symbole du gène STAT3
Identification du gène (NCBI) 6774
Conjugaison CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a mouse monoclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.

Protocole

Product Specific Protocols
FC protocol for CL555 STAT3 antibody CL555-60199Download protocol
Standard Protocols
Click here to view our Standard Protocols