Anticorps Polyclonal de lapin anti-STAT5A/B

• Phare
• Validé par KD/KO

STAT5A/B Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 13179-1-AP

Synonymes

MGF, STAT5, STAT5A

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Galerie de données de validation

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  WB analysis of Daudi using 13179-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13179-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A/B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 13179-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 13179-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13179-1-PBS

  Various lysates were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of 3T3-L1 using 13179-1-AP

  IP Result of anti-STAT5A/B (IP:13179-1-AP, 4ug; Detection:13179-1-AP 1:1000) with 3T3-L1 cells lysate 3200ug.

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  IHC staining of human breast cancer using 13179-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13179-1-AP (STAT5A/B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13179-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13179-1-AP (STAT5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13179-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13179-1-AP (STAT5A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13179-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13179-1-AP (STAT5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13179-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13179-1-AP (STAT5A/B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 13179-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13179-1-AP (STAT5A antibody) at dilution of 1:100 (under 40x lens).

• 

  FC experiment of MCF-7 using 13179-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug STAT5A/B antibody (13179-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules 3T3-L1, cellules DU 145, cellules K-562, cellules SGC-7901
• Résultats positifs en IP: cellules 3T3-L1
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

13179-1-AP cible STAT5A/B dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris, Vache
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STAT5A/B Protéine recombinante Ag3846
• Nom complet: signal transducer and activator of transcription 5A
• Masse moléculaire calculée: 794 aa, 92 kDa
• Poids moléculaire observé: 90-95 kDa, 50-55 kDa
• Numéro d’acquisition GenBank: BC027036
• Symbole du gène: STAT5A
• Identification du gène (NCBI): 6776
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells. This antibody is a rabbit polyclonal antibody raised against 445-794 aa of human STAT5A, the homology between this segment and the corresponding segment of human STAT5B is 92%. It shows that this antibody can cross-react with STAT5B.

Publications

Species	Application	Title
rat	WB,IF	Chemosphere; MEHP promotes liver fibrosis by down-regulating STAT5A in BRL-3A hepatocytes.; Authors - Yuezhu Zhang ; KD Validated
human	WB	Endocrinology; A Novel Mechanism of hPRL-G129R, A Prolactin Antagonist, Inhibits Human Breast Cancer Cell Proliferation and Migration; Authors - Ruonan Li
human	IHC,WB	Front Oncol; In-depth analysis of the expression and functions of signal transducers and activators of transcription in human ovarian cancer; Authors - Xiaodi Gong
rat	WB	Ecotoxicol Environ Saf; Mono-2-ethylhexyl phthalate (MEHP) promoted lipid accumulation via JAK2/STAT5 and aggravated oxidative stress in BRL-3A cells.; Authors - Yuezhu Zhang
mouse	WB	J Cell Mol Med; IL-27 Rα+ cells promoted allorejection via enhancing STAT1/3/5 phosphorylation.; Authors - Shanshan Zhao
human	WB	Cancer Cell Int; LNK promotes the growth and metastasis of triple negative breast cancer via activating JAK/STAT3 and ERK1/2 pathway.; Authors - Jianxin Lv

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