Anticorps Polyclonal de lapin anti-STAT6

• Phare
• Validé par KD/KO

STAT6 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

21

N° de cat : 51073-1-AP

Synonymes

D12S1644, IL 4 STAT, STAT6, STAT6B, STAT6C

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 51073-1-AP

  WB result of STAT6 antibody (51073-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT6 transfected NIH/3T3 cells.

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  WB analysis of NIH/3T3 using 51073-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 51073-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 51073-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules PC-12, cellules THP-1
• Résultats positifs en IHC: tissu splénique humain, tissu de cancer du côlon humain, tissu pulmonaire de souris, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

51073-1-AP cible STAT6 dans les applications de WB, RIP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: signal transducer and activator of transcription 6, interleukin-4 induced
• Masse moléculaire calculée: 94 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC075852
• Symbole du gène: STAT6
• Identification du gène (NCBI): 6778
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling. The antibody could identify about 100 kDa band, similar to the reported in the literature(PMID: 10961983).

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; miR-182 targeting reprograms tumor-associated macrophages and limits breast cancer progression.; Authors - Chengxin Ma
human,mouse	WB	Cell Rep; Macrophage autophagy deficiency-induced CEBPB accumulation alleviates atopic dermatitis via impairing M2 polarization; Authors - Yongcheng Zhu
human	IF	Front Cell Infect Microbiol; Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection.; Authors - Tian Luo
human	WB	Front Cell Dev Biol; A miR-129-5P/ARID3A Negative Feedback Loop Modulates Diffuse Large B Cell Lymphoma Progression and Immune Evasion Through Regulating the PD-1/PD-L1 Checkpoint; Authors - Weili Zheng
human	WB	Cells; Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer.; Authors - Damian Kołat
mouse,rat	WB,IHC	Life Sci; Berberine regulates macrophage polarization through IL-4-STAT6 signaling pathway in Helicobacter pylori-induced chronic atrophic gastritis.; Authors - Tao Yang