Anticorps Polyclonal de lapin anti-STC1

• Phare
• Validé par KD/KO

STC1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, ELISA, Cell treatment

Conjugaison

Non conjugué

Publications(14)

N° de cat : 20621-1-AP

Synonymes

Stanniocalcin-1, STC, STC-1

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Galerie de données de validation

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  WB analysis using 20621-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20621-1-AP (STC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 20621-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20621-1-AP (STC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 20621-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20621-1-AP (STC1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules SW480, cellules U-251
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 6 publications below
• IP: See 1 publications below

Informations sur le produit

20621-1-AP cible STC1 dans les applications de WB, IHC, IP, ELISA, Cell treatment et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STC1 Protéine recombinante Ag14697
• Nom complet: stanniocalcin 1
• Masse moléculaire calculée: 247 aa, 28 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC029044
• Symbole du gène: STC1
• Identification du gène (NCBI): 6781
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STC1, also named as stanniocalcin 1, a 56kDa homodimeric glycoprotein hormone, is highly expressed in a variety of cancers such as breast, ovarian, and colorectal cancers (PMID: 27459971). This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC1 is implicated in several pathologies including retinal degeneration, cerebral ischemia, angiogenesis and inflammation (PMID: 25391215). The calculated molecular weight of STC1 is usually approximately 28 kda.

Publications

Species	Application	Title
human	IHC	Inflamm Bowel Dis; Pro-inflammatory Cytokines Promote the Occurrence and Development of Colitis-associated Colorectal Cancer by Inhibiting miR-615-5p; Authors - Danping Sun
human	WB	Life Sci; Adiponectin alleviates the symptoms of ischemic renal disease by inhibiting renal cell apoptosis.; Authors - Xiaotong Zheng
human	WB	Cancer Cell Int; PMID: 31889892; Authors - Chuanfeng Zhang
human	WB	Neurochem Res; Stanniocalcin-1 Protected Astrocytes from Hypoxic Damage Through the AMPK Pathway.; Authors - Binda Sun ; KD Validated
human	IHC	Oncol Lett; Stanniocalcin 1 is a prognostic biomarker in glioma.; Authors - Weijian Luo
human	WB	Cancer Biother Radiopharm; Sevoflurane Suppresses Glioma Cell Proliferation, Migration, and Invasion Both In Vitro and In Vivo Partially Via Regulating KCNQ1OT1/miR-146b-5p/STC1 Axis.; Authors - Jian Wen

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