Anticorps Polyclonal de lapin anti-STEAP2-Specific

• Phare
• Validé par KD/KO

STEAP2-Specific Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 20201-1-AP

Synonymes

IPCA1, Metalloreductase STEAP2, PCANAP1, PUMPCn, STAMP1, STEAP2, STEAP2-Specific, STMP

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Galerie de données de validation

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  WB analysis of HEK-293 using 20201-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20201-1-AP

  WB result of STEAP2-Specific antibody (20201-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-STEAP2-Specific transfected HeLa cells.

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  WB analysis of DU 145 using 20201-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 20201-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20201-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20201-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules DU 145, cellules HeLa, tissu cérébral humain fœtal, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

20201-1-AP cible STEAP2-Specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: six transmembrane epithelial antigen of the prostate 2
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: NM_152999
• Symbole du gène: STEAP2
• Identification du gène (NCBI): 261729
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STEAP2, also named as PCANAP1 and STAMP1, belongs to the STEAP family. It is a metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. STEAP2 uses NAD+ as acceptor. The antibody is specific to STEAP2.

Publications

Species	Application	Title
human	WB	Exp Cell Res; Membrane cholesterol modulates STEAP2 conformation during dynamic intracellular trafficking processes leading to broad subcellular distribution.; Authors - Haruki Hasegawa
human	WB,IHC	Cell Death Dis; METTL3-mediated m6A modification of STEAP2 mRNA inhibits papillary thyroid cancer progress by blocking the Hedgehog signaling pathway and epithelial-to-mesenchymal transition.; Authors - Yue Zhu ; KD Validated
human	WB	Cancer Biol Ther; STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2; Authors - Dong Zhang ; KD Validated