Anticorps Recombinant de lapin anti-STEAP2

STEAP2 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240611E5

N° de cat : 83611-3-RR

Synonymes

STEAP2-Specific, Protein up-regulated in metastatic prostate cancer, Prostate cancer-associated protein 1, PCANAP1, Metalloreductase STEAP2

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Galerie de données de validation

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  WB analysis using 83611-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83611-3-RR (STEAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 83611-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STEAP2-Specific antibody (83611-3-RR, Clone: 240611E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HEK-293 using 83611-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STEAP2 antibody (83611-3-RR, Clone: 240611E5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of A549 using 83611-3-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug STEAP2-Specific Recombinant antibody (83611-3-RR, Clone:240611E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HEK-293 using 83611-3-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug STEAP2-Specific Recombinant antibody (83611-3-RR, Clone:240611E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules MCF-7, cellules U2OS
• Résultats positifs en IF/ICC: cellules HEK-293,
• Résultats positifs en FC (Intra): cellules HEK-293, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83611-3-RR cible STEAP2 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: six transmembrane epithelial antigen of the prostate 2
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 48-52 kDa
• Numéro d’acquisition GenBank: NM_152999
• Symbole du gène: STEAP2
• Identification du gène (NCBI): 261729
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STEAP2, also named as PCANAP1 and STAMP1, belongs to the STEAP family. It is a metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. STEAP2 uses NAD+ as acceptor.

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