Anticorps Polyclonal de lapin anti-STEAP4

• Phare
• Validé par KD/KO

STEAP4 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

32

Avis (1)

N° de cat : 11944-1-AP

Synonymes

Metalloreductase STEAP4, STAMP2, STEAP family member 4, STEAP4, TIARP, TNFAIP9

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Galerie de données de validation

• 

  WB analysis using 11944-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11944-1-AP (STEAP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 11944-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 11944-1-AP (STEAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11944-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11944-1-AP (STEAP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 11944-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 11944-1-AP (STEAP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11944-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11944-1-AP (STEAP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 11944-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11944-1-AP (STEAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of 3T3-L1 using 11944-1-AP

  IP result of anti-STEAP4 (IP:11944-1-AP, 4ug; Detection:11944-1-AP 1:1000) with 3T3-L1 cells lysate 1200 ug.

• 

  IP experiment of 3T3-L1 using 11944-1-AP

  IP result of anti-STEAP4(IP:11944-1-AP, 4ug; Detection:11944-1-AP 1:500) with 3T3-L1 cells lysate 1360 ug.

• 

  IHC staining of human prostate cancer using 11944-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11944-1-AP (STEAP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules 3T3-L1, cellules RAW 264.7, tissu cérébral humain, tissu hépatique de rat, tissu hépatique humain
• Résultats positifs en IP: cellules 3T3-L1,
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 25 publications below
• IHC: See 16 publications below
• IF: See 2 publications below

Informations sur le produit

11944-1-AP cible STEAP4 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STEAP4 Protéine recombinante Ag2545
• Nom complet: STEAP family member 4
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC020600
• Symbole du gène: STEAP4
• Identification du gène (NCBI): 79689
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STEAP4 (also called STAMP2), is a member of the STEAP family. Structurally, It is a 459 amino acid protein characterized by a molecular topology of six transmembrane domains. The cytoplasmic N-terminal domain of STEAP4 shows structural similarity with bacterial and archaeal FNO oxidoreductase and STEAP4 exhibits a strong iron reductase activity. Studies in mice and human suggest that STEAP4 maybe involved in adipocyte development and metabolism, and may contribute to the normal biology of the prostate cell, as well as prostate cancer progression.

Publications

Species	Application	Title
human	IHC	J Allergy Clin Immunol; Six-transmembrane epithelial antigens of the prostate comprise a novel inflammatory nexus in patients with pustular skin disorders.; Authors - Yun Liang
mouse,rat	WB,IHC	Bone Res; Dietary fat-associated osteoarthritic chondrocytes gain resistance to lipotoxicity through PKCK2/STAMP2/FSP27.; Authors - Sung Won Lee ; KD Validated
mouse,human,rat	IHC,WB	Basic Res Cardiol; The six-transmembrane protein Stamp2 ameliorates pulmonary vascular remodeling and pulmonary hypertension in mice.; Authors - Mehreen Batool ; KO Validated
human	WB,IHC	Nat Commun; Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis.; Authors - Yun Liao
mouse	IF	Proc Natl Acad Sci U S A; Quantitative proteomics identifies STEAP4 as a critical regulator of mitochondrial dysfunction linking inflammation and colon cancer.; Authors - Xiang Xue
human, mouse	WB, IHC	J Hepatol; PMID: 25646886; Authors - Hye Y Kim ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xiang (Verified Customer) (11-08-2019)

Works pretty good.

• Applications: Western Blot, Immunofluorescence,

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