Anticorps Polyclonal de lapin anti-STIM2

• Phare
• Validé par KD/KO

STIM2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 21192-1-AP

Synonymes

KIAA1482, STIM2, stromal interaction molecule 2

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Galerie de données de validation

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  WB analysis using 21192-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21192-1-AP (STIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21192-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21192-1-AP (STIM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21192-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21192-1-AP (STIM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 21192-1-AP

  IP Result of anti-STIM2 (IP:21192-1-AP, 3ug; Detection:21192-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IHC staining of mouse spleen using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21192-1-AP (STIM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21192-1-AP (STIM2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21192-1-AP (STIM2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 21192-1-AP (STIM2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 21192-1-AP (STIM2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21192-1-AP (STIM2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21192-1-AP (STIM2 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu splénique de souris, tissu cérébral humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

21192-1-AP cible STIM2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STIM2 Protéine recombinante Ag15530
• Nom complet: stromal interaction molecule 2
• Masse moléculaire calculée: 754 aa, 85 kDa
• Poids moléculaire observé: 85-115 kDa
• Numéro d’acquisition GenBank: BC146661
• Symbole du gène: STIM2
• Identification du gène (NCBI): 57620
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STIM2, also named as KIAA1482, functions as a highly sensitive Ca2+ sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca2+-influx. Regulates basal cytosolic and endoplasmic reticulum Ca2+ concentrations. Upon mild variations of the endoplasmic reticulum Ca2+ concentration, STIM2 translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca2+ release-activated Ca2+ (CRAC) channels ORAI1, ORAI2 and ORAI3. STIM2 may inhibit STIM1-mediated Ca2+ influx. STIM2 has 2 isoforms 105kd and 115kd with Differential levels of phosphorylation.

Publications

Species	Application	Title
mouse	WB	Cell Res; Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate.; Authors - Ao Hu ; KO Validated
human,mouse	WB,IF	Cell Mol Life Sci; STIM2 regulates NMDA receptor endocytosis that is induced by short-term NMDA receptor overactivation in cortical neurons; Authors - Karolina Serwach ; KD Validated
human	WB	Oncotarget; The calcium channel proteins ORAI3 and STIM1 mediate TGF-β induced Snai1 expression.; Authors - Atrayee Bhattacharya ; KD Validated
mouse	WB	Neurobiol Dis; Inhibiting store-operated calcium entry attenuates white matter secondary degeneration following SCI.; Authors - Ben C Orem
		Biochem J; STIM1 negatively regulates Ca2+ release from the sarcoplasmic reticulum in skeletal myotubes.; Authors - Lee Keon Jin KJ
mouse	WB	Cell Signal; RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate proinflammatory gene expression in microglia.; Authors - Menbere Wendimu ; KD Validated