Anticorps Polyclonal de lapin anti-STK38

STK38 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 55335-1-AP

Synonymes

Nuclear Dbf2-related kinase 1, Nuclear Dbf2 related kinase 1, NDR1 protein kinase, NDR1, NDR

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Galerie de données de validation

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  WB analysis of MCF-7 using 55335-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 55335-1-AP (STK38 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55335-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55335-1-AP (STK38 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 55335-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 55335-1-AP (STK38 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 55335-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55335-1-AP (STK38 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 55335-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55335-1-AP (STK38 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 55335-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55335-1-AP (STK38 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 55335-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55335-1-AP (STK38 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 55335-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STK38 antibody (55335-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

55335-1-AP cible STK38 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: serine/threonine kinase 38
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: NM_007271
• Symbole du gène: STK38
• Identification du gène (NCBI): 11329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Serine/threonine-protein kinase 38 belongs to AGC Ser/Thr protein kinase family.STK38 implicates in cell proliferation and/or tumor progression, and might play a role in the HIV-1 life cycle.Researches showed that STK38 with STK38L, were proapoptotic kinases and key members of the RASSF1/STK4 signaling cascade and its kinase activity increased in early mitotic phase and was dependent on FRY and STK3.This antibody recognizes the C-terminal of STK38.

Publications

Species	Application	Title
human	IF, WB, IHC	J Cell Biochem; Phosphorylated LASS2 inhibits prostate carcinogenesis via negative regulation of Wnt/β-catenin signaling.; Authors - Kuangen Zhang
human	WB,IF	J Proteome Res; ZSWIM1 Promotes the Proliferation and Metastasis of Lung Adenocarcinoma Cells through the STK38/MEKK2/ERK1/2 Axis; Authors - Xuejuan Gao
human	WB,IP	Nat Commun; A bacterial autotransporter impairs innate immune responses by targeting the transcription factor TFE3; Authors - Atri Ta

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