Anticorps Recombinant de lapin anti-STK39

STK39 Recombinant Antibody for IF/ICC, IF-P

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

IF/ICC, IF-P

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230382G9

N° de cat : CL488-83081-2

Synonymes

230382G9, DCHT, PASK, SPAK, Ste 20 related kinase

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Galerie de données de validation

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  IF Staining of mouse brain using CL488-83081-2

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 STK39 antibody (CL488-83081-2, Clone: 230382G9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using CL488-83081-2

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CoraLite® Plus 488 STK39 antibody (CL488-83081-2, Clone: 230382G9 ) at dilution of 1:200.

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  FC experiment of Daudi using CL488-83081-2

  1x10^6 Daudi cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Stk39 Recombinant Antibody (CL488-83081-2, Clone:230382G9)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules NIH/3T3,
• Résultats positifs en FC (Intra): cellules Daudi,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-83081-2 cible STK39 dans les applications de IF/ICC, IF-P et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: STK39 Protéine recombinante Ag34503
• Nom complet: serine threonine kinase 39 (STE20/SPS1 homolog, yeast)
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC166614
• Symbole du gène: STK39
• Identification du gène (NCBI): 27347
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STK39 (Serine/Threonine Kinase 39) is a proline- and alanine-rich Ste20-related kinase (also named as SPAK), which is composed of a short N-terminal proline and alanine repeats (PAPA box), a kinase catalytic domain and a C-terminal regulatory domain (PMID: 16382158). In mammalians, STK39 plays an important role in ion homeostasis by regulating the cation chloride cotransporters' activities, which is crucial for the modulation of renal salt transport and blood pressure (PMID: 33500714). Notably, STK39 is also implicated in the regulation of tumor cell proliferation, migration, and invasion in multiple cancers, including osteosarcoma and cervical cancer (PMID: 34335956).

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