Anticorps Polyclonal de lapin anti-MST1

• Phare
• Validé par KD/KO

MST1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 22245-1-AP

Synonymes

KRS2, MST 1, MST1, serine/threonine kinase 4, STE20 like kinase MST1, STK4, STK4/MST1, YSK3

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Galerie de données de validation

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  WB analysis of HepG2 using 22245-1-AP

  WB result of MST1 antibody (22245-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MST1 transfected HepG2 cells.

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  WB analysis using 22245-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22245-1-AP (MST1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 22245-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 22245-1-AP (MST1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22245-1-AP

  IP Result of anti-MST1 (IP:22245-1-AP, 4ug; Detection:22245-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of human prostate cancer using 22245-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22245-1-AP (MST1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 22245-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22245-1-AP (MST1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 22245-1-AP

  Immunofluorescent analysis of HepG2 cells using 22245-1-AP (MST1 antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C2C12, cellules HepG2, cellules Jurkat, cellules Ramos, tissu hépatique de rat, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 22 publications below
• IHC: See 7 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

22245-1-AP cible MST1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MST1 Protéine recombinante Ag17738
• Nom complet: serine/threonine kinase 4
• Masse moléculaire calculée: 487 aa, 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC093768
• Symbole du gène: STK4
• Identification du gène (NCBI): 6789
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases . STK3/4 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the STK3/4 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. STK4 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of STK4 at Thr183 (Thr180 in STK3) in the activation loop is a key activation mechanism for STK4/3 because phosphorylation of Thr183/180 causes the cleavage of STK4 by caspases under apoptotic conditions. The caspase-cleavage results in a more active STK4 protein (STK4-N, an amino-terminally truncated STK4), which localizes into the nucleus and induces apoptosis through histone modifications and chromatin condensations.

Publications

Species	Application	Title
mouse	IF,WB	Cell; Glycogen accumulation and phase separation drives liver tumor initiation.; Authors - Qingxu Liu
human	WB	Nat Commun; Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.; Authors - Shujie Chen
mouse	IHC	Cell Death Differ; Hippo kinases MST1 and MST2 control the differentiation of the epididymal initial segment via the MEK-ERK pathway.; Authors - Chenling Meng
mouse	WB,IHC	J Am Soc Nephrol; Tubule-Specific Mst1/2 Deficiency Induces CKD via YAP and Non-YAP Mechanisms.; Authors - Chunhua Xu ; KO Validated
human	WB,IHC,IF	Oncogene; Deacetylation of tumor-suppressor MST1 in Hippo pathway induces its degradation through HBXIP-elevated HDAC6 in promotion of breast cancer growth.; Authors - L Li
human	WB	Cell Death Differ; Suppressor of hepatocellular carcinoma RASSF1A activates autophagy initiation and maturation.; Authors - Wenjiao Li