Anticorps Polyclonal de lapin anti-STOML2

• Phare
• Validé par KD/KO

STOML2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

47

Avis (3)

N° de cat : 10348-1-AP

Synonymes

EPB72 like protein 2, HSPC108, SLP 2, SLP2, stomatin (EPB72) like 2, Stomatin like protein 2, STOML2

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Galerie de données de validation

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  WB analysis using 10348-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10348-1-AP (STOML2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10348-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10348-1-AP (STOML2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10348-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10348-1-AP (STOML2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10348-1-AP

  IP Result of anti-STOML2 (IP:10348-1-AP, 3ug; Detection:10348-1-AP 1:1000) with mouse brain tissue lysate 1000ug.

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  IHC staining of human lung cancer using 10348-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10348-1-AP (STOML2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 10348-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10348-1-AP (STOML2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 10348-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10348-1-AP (STOML2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 10348-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10348-1-AP (STOML2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human endometrial cancer using 10348-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 10348-1-AP (STOML2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human endometrial cancer using 10348-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 10348-1-AP (STOML2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 10348-1-AP

  Immunofluorescent analysis of MCF-7 cells, using STOML2 antibody 10348-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 10348-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STOML2 antibody (10348-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules C6, cellules HEK-293, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de l'endomètre humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 37 publications below
• IHC: See 23 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Informations sur le produit

10348-1-AP cible STOML2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STOML2 Protéine recombinante Ag0363
• Nom complet: stomatin (EPB72)-like 2
• Masse moléculaire calculée: 356 aa, 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC002442
• Symbole du gène: STOML2
• Identification du gène (NCBI): 30968
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STOML2 (Stomatin-like protein 2; also known as SLP-2) is a widely expressed mitochondrial member of the highly conserved family of stomatin proteins. STOML2 is localized mostly in mitochondrial inner membrane, minor in plasma membrane. Human STOML2 interacts with prohibitins and regulates mitochondrial biogenesis and function (21746876). In addition, plenty of studies revealed that STOML2 was overexpressed in many human cancer tissues, and its expression may be a new valuable prognostic biomarker (19839737, 21960069). Recently STOML2 has been identified as a serological biomarker for early colorectal cancer (CRC) diagnosis (21209152). Two isoforms of STOML2 exist due to the alternative splicing, with MW of 39 kDa and 33 kDa, respectively.

Publications

Species	Application	Title
human	WB	Cell Metab; DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.; Authors - Ricarda Richter-Dennerlein
human	WB,IHC	Clin Cancer Res; Stomatin-like protein 2 is overexpressed in cancer and involved in regulating cell growth and cell adhesion in human esophageal squamous cell carcinoma.; Authors - Zhang Liyong L
human	WB	EMBO Rep; The membrane scaffold SLP2 anchors a proteolytic hub in mitochondria containing PARL and the i-AAA protease YME1L.; Authors - Timothy Wai
human	WB	Hum Mol Genet; Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.; Authors - McCray Brett A BA
human	WB	J Pathol; Knockdown of stomatin-like protein 2 (STOML2) reduces the invasive ability of glioma cells through inhibition of the NF-κB/MMP-9 pathway.; Authors - Song Libing L
human	WB,IHC	Mol Cell Proteomics; An informatics-assisted label-free approach for personalized tissue membrane proteomics: case study on colorectal cancer.; Authors - Han Chia-Li CL

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Gaurav (Verified Customer) (02-15-2024)

Good antibody which works as per the description. Detects the endogenous proteins without any problem.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293, Smooth Muscle Cells

STOML2 Antibody Western Blot,Immunoprecipitation validation (1:3000 dilution) in HEK293, Smooth Muscle Cells (Cat no:10348-1-AP)

FH

Hussein (Verified Customer) (03-23-2021)

I validated the antibody specificity using different siRNAs. The signal is strong in IB and bright in IF and IHC.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: WB 1:1000 (each dilution was used 3 times); IP 1:200; IHC 1:400
• Cell Tissue Type: Breast cancer cell lines: MDA-MB-231, MDA-MB-361, BT-474, JIMT-1

FH

SITING (Verified Customer) (08-31-2020)

excellent antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: heart

STOML2 Antibody Western Blot validation (1:1000 dilution) in heart (Cat no:10348-1-AP)

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