Anticorps Polyclonal de lapin anti-STS

• Phare
• Validé par KD/KO

STS Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 17870-1-AP

Synonymes

ARSC, ARSC1, Arylsulfatase C, ASC, ES, SSDD, Steroid sulfatase, Steryl sulfatase, Steryl sulfate sulfohydrolase, STS

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Galerie de données de validation

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  WB analysis of MCF-7 using 17870-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17870-1-AP (STS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 17870-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17870-1-AP (STS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17870-1-AP (STS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17870-1-AP (STS antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17870-1-AP (STS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17870-1-AP (STS antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17870-1-AP (STS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17870-1-AP (STS antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17870-1-AP (STS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hysteromyoma using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 17870-1-AP (STS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hysteromyoma using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 17870-1-AP (STS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human paracancerous tissue of hysteromyoma using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human paracancerous tissue of hysteromyoma using 17870-1-AP (STS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human paracancerous tissue of hysteromyoma using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human paracancerous tissue of hysteromyoma using 17870-1-AP (STS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17870-1-AP (STS antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17870-1-AP (STS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17870-1-AP (STS antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 17870-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 17870-1-AP (STS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF7, tissu placentaire humain
• Résultats positifs en IHC: tissu placentaire humain, tissu cutané humain, tissu d'hystéromyome humain, tissu ovarien humain, tissu pulmonaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

17870-1-AP cible STS dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STS Protéine recombinante Ag12353
• Nom complet: steroid sulfatase (microsomal), isozyme S
• Masse moléculaire calculée: 583 aa, 65 kDa
• Poids moléculaire observé: 63-65 kDa
• Numéro d’acquisition GenBank: BC075030
• Symbole du gène: STS
• Identification du gène (NCBI): 412
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STS(Steroid sulfatase) is also named as ASC(arylsulfatase C), ARSC1 and belongs to the sulfatase family. It is expressed in human liver and is responsible for the hydrolysis of many estrogen and hydroxysteroid sulfates, including dehydroepiandrosterone (DHEA)-sulfate and β-estradiol(E2)-3-sulfate(PMID:19589875). This enzyme has also been documented to occur in many other tissues, including skin, lung, ovary, and adrenal gland. The estimated molecular mass of expressed human STS is 63-85 kDa which may represent different levels of glycosylation in the different tissues, as STS is known to be a glycoprotein and the full length protein has a signal peptide with 21 amino acids(PMID:17604157).

Publications

Species	Application	Title
human	WB	Clin Cancer Res; Steroid Sulfatase Stimulates Intracrine Androgen Synthesis and is a Therapeutic Target for Advanced Prostate Cancer.; Authors - Cameron M Armstrong ; KD Validated
mouse, rat	WB	Sci Rep; Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application.; Authors - Jiansha Li
human	WB	Mol Cell Biochem; Resveratrol and its methoxy derivatives modulate the expression of estrogen metabolism enzymes in breast epithelial cells by AhR down-regulation.; Authors - Barbara Licznerska
mouse	WB,IF	J Biomed Res; Suppressed estrogen supply via extra-ovarian progesterone receptor membrane component 1 in menopause.; Authors - Sang R Lee
mouse	IF	Life Sci Alliance; Macrophage Sult2b1 promotes pathological neovascularization in age-related macular degeneration; Authors - Yafang Wang