Anticorps Polyclonal de lapin anti-STX17

• Phare
• Validé par KD/KO

STX17 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(56)

Avis (1)

N° de cat : 17815-1-AP

Synonymes

Syntaxin 17, Syntaxin-17

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Galerie de données de validation

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  WB analysis of HepG2 using 17815-1-AP

  WB result of STX17 antibody (17815-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STX17 transfected HepG2 cells.

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  WB analysis using 17815-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17815-1-AP (STX17 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 17815-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 17815-1-AP (STX17 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 17815-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 17815-1-AP (STX17 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 17815-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17815-1-AP (STX17 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17815-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17815-1-AP (STX17 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17815-1-AP

  IP result of anti-STX17 (IP:17815-1-AP, 4ug; Detection:17815-1-AP 1:1000) with HepG2 cells lysate 3600ug.

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  IHC staining of mouse testis using 17815-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17815-1-AP (STX17 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17815-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17815-1-AP (STX17 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 17815-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17815-1-AP (STX17 antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A375, cellules Jurkat, cellules L02, tissu testiculaire de rat, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu testiculaire de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 43 publications below
• IHC: See 4 publications below
• IF: See 16 publications below
• IP: See 6 publications below
• CoIP: See 4 publications below

Informations sur le produit

17815-1-AP cible STX17 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STX17 Protéine recombinante Ag12134
• Nom complet: syntaxin 17
• Masse moléculaire calculée: 302 aa, 33 kDa
• Poids moléculaire observé: 33-38 kDa
• Numéro d’acquisition GenBank: BC111009
• Symbole du gène: STX17
• Identification du gène (NCBI): 55014
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for the fusion of cellular membranes. Syntaxin 17 (STX17) is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. STX17 may also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC, and the Golgi.

Publications

Species	Application	Title
human	WB	Cancer Cell; Cancer cells escape autophagy inhibition via NRF2-induced macropinocytosis.; Authors - Hua Su
human	WB,IF,IP	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB,IF	Autophagy; Atractylenolide I inhibits angiogenesis and reverses sunitinib resistance in clear cell renal cell carcinoma through ATP6V0D2-mediated autophagic degradation of EPAS1/HIF2α; Authors - Qinyu Li
human	WB	Autophagy; DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B.; Authors - Xiaoyu Tian
mouse	WB	Autophagy; PMID: 32432943; Authors - Zu-Guo Zheng ; KD Validated
human	WB	Autophagy; Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress; Authors - Jia-min Yan ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jane (Verified Customer) (01-07-2022)

No working, non specific bands.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Heart/Cardiomyocytes

STX17 Antibody Western Blot,Immunohistochemistry validation (1:500 dilution) in Heart/Cardiomyocytes (Cat no:17815-1-AP)

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