Anticorps Polyclonal de lapin anti-Syntaxin 4

Syntaxin 4 Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 14988-1-AP

Synonymes

p35 2, STX4, STX4A, syntaxin 4

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Galerie de données de validation

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  WB analysis using 14988-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14988-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14988-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 14988-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14988-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 14988-1-AP

  IP Result of anti-Syntaxin 4 (IP:14988-1-AP, 4ug; Detection:14988-1-AP 1:1500) with mouse kidney tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HEK-293, cellules Jurkat, tissu rénal de rat
• Résultats positifs en IP: tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below

Informations sur le produit

14988-1-AP cible Syntaxin 4 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Syntaxin 4 Protéine recombinante Ag6973
• Nom complet: syntaxin 4
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 35-37 kDa
• Numéro d’acquisition GenBank: BC002436
• Symbole du gène: STX4
• Identification du gène (NCBI): 6810
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Brain; Reduced oligodendrocyte exosome secretion in multiple system atrophy involves SNARE dysfunction.; Authors - Zhenwei Yu
human	WB	Front Immunol; Bi-Allelic Mutations in STXBP2 Reveal a Complementary Role for STXBP1 in Cytotoxic Lymphocyte Killing.; Authors - Jamie A Lopez
human	WB	Int J Biol Macromol; PKCε controls the fusion of secretory vesicles in mast cells in a phosphatidic acid-dependent mode.; Authors - Emilio M Serrano-López
human	WB	Br J Pharmacol; CRP promotes atherosclerosis by increasing LDL transcytosis across endothelial cells.; Authors - Fang Bian
human	WB	J Biol Chem; Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin.; Authors - Wulin Aerbajinai
human	WB	J Transl Med; Angiopoietin2-mediated caveolin1 phosphorylation regulating transcytosis of renal tubular epithelial cell contributes to the occurrence of albuminuria under high glucose exposure.; Authors - Jing Liu