Anticorps Monoclonal anti-Syntaxin 6

Syntaxin 6 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

2B6G4

N° de cat : 60059-1-Ig

Synonymes

2B6G4, STX6, Syntaxin-6

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Galerie de données de validation

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  WB analysis using 60059-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 60059-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 60059-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 60059-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 60059-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 60059-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 60059-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 60059-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-12 using 60059-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Syntaxin 6 antibody (60059-1-Ig, Clone: 2B6G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules C6, cellules Jurkat, cellules Neuro-2a, cellules SH-SY5Y, cellules U-251, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IHC: tissu cérébral humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-12,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

60059-1-Ig cible Syntaxin 6 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Syntaxin 6 Protéine recombinante Ag1284
• Nom complet: syntaxin 6
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC009944
• Symbole du gène: Syntaxin 6
• Identification du gène (NCBI): 10228
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.

Publications

Species	Application	Title
human	WB	Nat Commun; Suppression of ACE2 SUMOylation protects against SARS-CoV-2 infection through TOLLIP-mediated selective autophagy; Authors - Shouheng Jin
	WB	PLoS One; Expression and functional characterization of His-tagged prestin in Chinese hamster ovary cells; Authors - Ryosei Motoo

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