Anticorps Polyclonal de lapin anti-Syntaxin 8

Syntaxin 8 Polyclonal Antibody for FC, IF, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 12206-1-AP

Synonymes

CARB, STX8, syntaxin 8

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Galerie de données de validation

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  WB analysis of human heart using 12206-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12206-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12206-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12206-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12206-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12206-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 12206-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12206-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A375 using 12206-1-AP

  IP Result of anti-Syntaxin 8 (IP:12206-1-AP, 4ug; Detection:12206-1-AP 1:500) with A375 cells lysate 3600ug.

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  IF Staining of HepG2 using 12206-1-AP

  Immunofluorescent analysis of HepG2 cells, using STX8 antibody 12206-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 12206-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug Syntaxin 8 antibody (12206-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, cellules A375, tissu cérébral humain, tissu hépatique humain, tissu pancréatique de souris, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain
• Résultats positifs en IP: cellules A375
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

12206-1-AP cible Syntaxin 8 dans les applications de WB, IP, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Syntaxin 8 Protéine recombinante Ag2850
• Nom complet: syntaxin 8
• Masse moléculaire calculée: 236 aa, 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC009713
• Symbole du gène: STX8
• Identification du gène (NCBI): 9482
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Syntaxin 8 (STX8), also known as CIP-1-associated regulator of cyclin B (CARB ), is a protein belongs to the syntaxin family. The gene of STX8 maps to chromosomal band 17p12 and it encodes a 236-amino-acid protein containing 1 t-SNARE coiled-coil homology domain. STX8 mRNA is broadly expressed in multiple human tissues, including heart, brain, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas (PMID: 10198254). STX8 is involved in protein trafficking from early to late endosomes via vesicle fusion and exocytosis.

Publications

Species	Application	Title
rat	WB,CoIP	J Cell Biol; VAMP4 regulates insulin levels by targeting secretory granules to lysosomes; Authors - Min Li ; KD Validated
mouse		PLoS One; The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification.; Authors - Mona Abed