Anticorps Polyclonal de lapin anti-STXBP2

STXBP2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 15312-1-AP

Synonymes

Hunc18b, MUNC18 2, pp10122, STXBP2, STXBP2 protein, syntaxin binding protein 2, UNC18 2, UNC18B

---

Galerie de données de validation

• 

  WB analysis of K-562 using 15312-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15312-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15312-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15312-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 15312-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 15312-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15312-1-AP (STXBP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 15312-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15312-1-AP (STXBP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15312-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15312-1-AP (STXBP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15312-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15312-1-AP (STXBP2 antibody) at dilution of 1:50 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: cellules K-562, cellules Jurkat, tissu cérébral de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

15312-1-AP cible STXBP2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STXBP2 Protéine recombinante Ag7537
• Nom complet: syntaxin binding protein 2
• Masse moléculaire calculée: 593 aa, 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC002869
• Symbole du gène: STXBP2
• Identification du gène (NCBI): 6813
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Syntaxin-binding protein 2 (STXBP2, also known as Unc18-2) belongs to the STXBP/unc-18/SEC1 family. STXBP2 is involved in intracellular trafficking, control of SNARE (soluble NSF attachment protein receptor) complex assembly, and the release of cytotoxic granules by natural killer cells. Mutations in the STXBP2 gene are associated with familial hemophagocytic lymphohistiocytosis.

Publications

Species	Application	Title
human	WB	J Exp Med; Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.; Authors - Frank Cichocki
human	WB	J Cell Biol; The septin cytoskeleton regulates natural killer cell lytic granule release.; Authors - Prasad V Phatarpekar
human	WB	Front Immunol; An N-Terminal Missense Mutation in STX11 Causative of FHL4 Abrogates Syntaxin-11 Binding to Munc18-2.; Authors - Martha-Lena Müller
rat	WB	Neuroscience; SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.; Authors - Xing-Lian Duan
	WB	Int J Hematol; Molecular analysis of the novel L243R mutation in STXBP2 reveals impairment of degranulation activity.; Authors - Laura Viñas-Giménez

Produits associés

Syntaxin 1A / Syntaxin 1B Monoclonal antibody

66437-1-Ig

STXBP2 Monoclonal antibody

66238-1-Ig

Syntaxin 2 Polyclonal antibody

55033-1-AP

Syntaxin 3 Polyclonal antibody

15556-1-AP

STXBP1 Polyclonal antibody

11459-1-AP

STXBP1 Polyclonal antibody

20562-1-AP

STXBP3 Polyclonal antibody

13764-1-AP

STXBP4 Polyclonal antibody

14231-1-AP

STXBP6 Polyclonal antibody

10976-4-AP