Anticorps Polyclonal de lapin anti-SUCLA2

• Phare
• Validé par KD/KO

SUCLA2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

20

N° de cat : 12627-1-AP

Synonymes

A BETA, SCS betaA, SUCLA2

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Galerie de données de validation

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  WB analysis of HEK-293 using 12627-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12627-1-AP (SUCLA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12627-1-AP

  WB result of SUCLA2 antibody (12627-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUCLA2 transfected HepG2 cells.

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  WB analysis of L02 using 12627-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12627-1-AP (SUCLA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 12627-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12627-1-AP (SUCLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12627-1-AP

  IP Result of anti-SUCLA2 (IP:12627-1-AP, 4ug; Detection:12627-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

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  IHC staining of human colon cancer using 12627-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12627-1-AP (SUCLA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12627-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12627-1-AP (SUCLA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12627-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12627-1-AP (SUCLA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12627-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12627-1-AP (SUCLA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 12627-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12627-1-AP (SUCLA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 12627-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12627-1-AP (SUCLA2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 12627-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12627-1-AP (SUCLA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 12627-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12627-1-AP (SUCLA2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 12627-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 12627-1-AP (SUCLA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules L02, cellules PC-3, cellules SH-SY5Y, tissu cérébral de souris, tissu hépatique de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cardiaque humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 17 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

12627-1-AP cible SUCLA2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SUCLA2 Protéine recombinante Ag3319
• Nom complet: succinate-CoA ligase, ADP-forming, beta subunit
• Masse moléculaire calculée: 463 aa, 50 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC027587
• Symbole du gène: SUCLA2
• Identification du gène (NCBI): 8803
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SUCLA2, β subunit of succinate-CoA ligase, contributes to energy production in mitochondria through the succinate-CoA ligase enzyme. Succinate-CoA ligase catalyses the reversible conversion of succinyl-CoA and ADP or GDP to succinate and ATP or GTP. SUCLA2 mutations cause global protein succinylation and mitochondrial DNA depletion (PMID: 33230181, PMID: 20301762). Mutations in SUCLA2 are also associated with SUCLA2-related mitochondrial DNA depletion syndrome, leigh syndrome, down syndrome (PMID: 23010432).

Publications

Species	Application	Title
human	WB	Mol Cell; Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation; Authors - Sophie Trefely
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang
mouse	WB	Aging Dis; Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging; Authors - Minghao Yuan
human	WB,IHC	Oncogene; Pharmacologically targetable vulnerability in prostate cancer carrying RB1-SUCLA2 deletion.; Authors - Susumu Kohno ; KO Validated
human,mouse	WB,IHC	Oncogene; MUC1 triggers lineage plasticity of Her2 positive mammary tumors; Authors - Zhi Pang
human	IF	Brain Struct Funct; Exclusive neuronal expression of SUCLA2 in the human brain.; Authors - Dobolyi Arpád A