Anticorps Polyclonal de lapin anti-SULT1A3

SULT1A3 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 19499-1-AP

Synonymes

Aryl sulfotransferase 1A3/1A4, HAST, HAST3, M PST, ST1A3/ST1A4, ST1A5, STM, Sulfotransferase 1A3/1A4, SULT1A3, SULT1A4, TL PST

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Galerie de données de validation

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  WB analysis using 19499-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 19499-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 19499-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 19499-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 19499-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 19499-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 19499-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19499-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 19499-1-AP

  IP Result of anti-SULT1A3 (IP:19499-1-AP, 4ug; Detection:19499-1-AP 1:200) with COLO 320 cells lysate 2240ug.

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  IHC staining of human hepatocirrhosis using 19499-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 19499-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 19499-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19499-1-AP (SULT1A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19499-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19499-1-AP (SULT1A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19499-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 19499-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules Caco-2, cellules COLO 320, cellules HepG2, cellules HuH-7, cellules MCF-7, tissu hépatique de souris
• Résultats positifs en IP: cellules COLO 320
• Résultats positifs en IHC: tissu de cirrhose hépatique humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

19499-1-AP cible SULT1A3 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: sulfotransferase family, cytosolic, 1A, phenol-preferring, member 3
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 30-34 kDa
• Numéro d’acquisition GenBank: NM_177552
• Symbole du gène: SULT1A3
• Identification du gène (NCBI): 6818
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SULT1A3, also named as STM and SULT1A4, belongs to the sulfotransferase 1 family. SULT1A3 catalyzes the sulfate conjugation of phenolic monoamines (neurotransmitters such as dopamine, norepinephrine and serotonin) and phenolic and catechol drugs.

Publications

Species	Application	Title
human	IHC	Cancer Lett; Expression of sulfotransferases and sulfatases in human breast cancer: impact on resveratrol metabolism.; Authors - Miksits Michaela M
mouse	WB	J Adv Res; Endogenous hydrogen sulfide improves vascular remodeling through PPARδ/SOCS3 signaling.; Authors - Danyang Tian