Anticorps Polyclonal de lapin anti-SULT1E1

• Phare
• Validé par KD/KO

SULT1E1 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 12522-1-AP

Synonymes

EST, EST 1, Estrogen sulfotransferase, ST1E1, STE, Sulfotransferase 1E1, SULT1E1

---

Galerie de données de validation

• 

  WB analysis of human lung using 12522-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12522-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human adrenal gland using 12522-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12522-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12522-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 12522-1-AP

  IP Result of anti-SULT1E1 (IP:12522-1-AP, 4ug; Detection:12522-1-AP 1:400) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human breast cancer using 12522-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12522-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu pulmonaire humain, tissu de glande surrénale humain, tissu hépatique humain, tissu rénal de souris, tissu rénal humain
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

12522-1-AP cible SULT1E1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SULT1E1 Protéine recombinante Ag3216
• Nom complet: sulfotransferase family 1E, estrogen-preferring, member 1
• Masse moléculaire calculée: 294 aa, 35 kDa
• Poids moléculaire observé: 32-35 kDa
• Numéro d’acquisition GenBank: BC027956
• Symbole du gène: SULT1E1
• Identification du gène (NCBI): 6783
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SULT1E1, also known as estrogen sulfotransferase, catalyzes the sulfation of endogenous estrogens as well as xenobiotic estrogen-like chemicals, converting them into the inactive form. SULT1E1 is a 33-35 kDa cytosolic protein that has been detected in human hepatic and nonhepatic tissues (17035602). SULT1E1 is expressed in breast and endometrial tissues, and some studies have investigated its implication in tumor development. Higher expression of SULT1E1 was observed in breast cancer tissues compared with normal breast tissues (22380844). This antibody detected a major band around 35 kD in lysates of mouse kidney, human liver and so on.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Oestrogen sulfotransferase ablation sensitizes mice to sepsis.; Authors - Xiaojuan Chai
	IHC	J Am Soc Nephrol; Inhibition of Estrogen Sulfotransferase (SULT1E1/EST) Ameliorates Ischemic Acute Kidney Injury in Mice.; Authors - Anne C Silva Barbosa
human	WB	Biochem Pharmacol; PXR phosphorylated at Ser350 transduces a glucose signal to repress the estrogen sulfotransferase gene in human liver cells and fasting signal in mouse livers.; Authors - Hao Hu
mouse	WB	J Biol Chem; Estrogen sulfotransferase is an oxidative stress responsive gene that gender-specifically affects liver ischemia/reperfusion injury.; Authors - Yan Guo ; KO Validated
human	WB	Biochem Pharmacol; Farnesoid X receptor regulates SULT1E1 expression through inhibition of PGC1α binding to HNF4α.; Authors - Shuai Wang
mouse	WB	Endocrinology; PMID: 28938414; Authors - Wojciech G Garbacz