Anticorps Monoclonal anti-SUMO1

SUMO1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

3B5B5

Avis (1)

N° de cat : 67559-1-Ig

Synonymes

3B5B5, GAP-modifying protein 1, GMP1, OK/SW-cl.43, Sentrin

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Galerie de données de validation

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  WB analysis using 67559-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67559-1-Ig (SUMO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat kidney using 67559-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67559-1-Ig (SUMO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat small intestine using 67559-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 67559-1-Ig (SUMO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 67559-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67559-1-Ig (SUMO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 67559-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SUMO1 antibody (67559-1-Ig, Clone: 3B5B5 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of A549 using 67559-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SUMO1 antibody (67559-1-Ig, Clone: 3B5B5 ) at dilution of 1:3200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IHC: tissu rénal de souris, tissu d'intestin grêle de rat, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

67559-1-Ig cible SUMO1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SUMO1 Protéine recombinante Ag29402
• Nom complet: SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 15-100 kDa
• Numéro d’acquisition GenBank: BC006462
• Symbole du gène: SUMO1
• Identification du gène (NCBI): 7341
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.This antibody can detect endogenous levels of SUMOylated proteins (e.g. SUMO-1-RanGAP at 80-90 kD).

Publications

Species	Application	Title
mouse	IP	Biomed Pharmacother; Hydrogen sulfide regulates SERCA2a SUMOylation by S-Sulfhydration of SENP1 to ameliorate cardiac systole-diastole function in diabetic cardiomyopathy; Authors - Shuo Peng
human	WB	PLoS One; Isoginkgetin and Madrasin are poor splicing inhibitors; Authors - Michael Tellier
mouse	IF	Cell Biol Toxicol; SENP1 inhibits aerobic glycolysis in Aβ1-42-incubated astrocytes by promoting PUM2 deSUMOylation; Authors - Qianshuo Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mi (Verified Customer) (05-29-2023)

It works well in IP assay in human white adipocytes.

• Applications: Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: adipocytes

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