Anticorps Polyclonal de lapin anti-SUZ12

• Phare
• Validé par KD/KO

SUZ12 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

12

Avis (1)

N° de cat : 20366-1-AP

Synonymes

ChET 9 protein, CHET9, JJAZ1, Joined to JAZF1 protein, KIAA0160, Polycomb protein SUZ12, SUZ12

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Galerie de données de validation

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  WB analysis using 20366-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20366-1-AP (SUZ12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20366-1-AP

  WB result of SUZ12 antibody (20366-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUZ12 transfected HEK-293 cells.

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  IP experiment of HeLa using 20366-1-AP

  IP Result of anti-SUZ12 (IP:20366-1-AP, 4ug; Detection:20366-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IP experiment of HeLa using 20366-1-AP

  IP Result of anti-SUZ12 (IP:20366-1-AP, 4ug; Detection:20366-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human breast cancer using 20366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20366-1-AP (SUZ12 Antibody) at dilution of 1:400.

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  IHC staining of human breast cancer using 20366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20366-1-AP (SUZ12 Antibody) at dilution of 1:100.

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  IHC staining of human breast cancer using 20366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20366-1-AP (SUZ12 Antibody) at dilution of 1:100.

• 

  IHC staining of human breast cancer using 20366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20366-1-AP (SUZ12 Antibody) at dilution of 1:200.

• 

  IHC staining of human breast cancer using 20366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20366-1-AP (SUZ12 Antibody) at dilution of 1:200.

• 

  IHC staining of human breast cancer using 20366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20366-1-AP (SUZ12 Antibody) at dilution of 1:400.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules MCF-7
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

20366-1-AP cible SUZ12 dans les applications de WB, IP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: suppressor of zeste 12 homolog (Drosophila)
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 80-90 kDa
• Numéro d’acquisition GenBank: NM_015355
• Symbole du gène: SUZ12
• Identification du gène (NCBI): 23512
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SUZ12, also termed CHET9, JJAZ1 or KIAA0160, belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. It is a polycomb group (PcG) protein. SUZ12 is a component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors.

Publications

Species	Application	Title
human	WB	Mol Cell; TRACE generates fluorescent human reporter cell lines to characterize epigenetic pathways.; Authors - Iva A Tchasovnikarova ; KD Validated
human	WB	EMBO J; A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.; Authors - Xu Wang
human	WB	Eur J Med Chem; Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.; Authors - Cheng Wang
human	WB	Sci Rep; Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1.; Authors - Masamitsu N Asaka
human	WB	Am J Cancer Res; N-terminal truncated carboxypeptidase E represses E-cadherin expression in lung cancer by stabilizing the Snail-HDAC complex.; Authors - Jing Sun
human	WB	Acta Pharmacol Sin; AZD9291 inactivates the PRC2 complex to mediate tumor growth inhibition.; Authors - Kai-Li Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nikhil (Verified Customer) (01-07-2022)

HCT116 whole cell extract, 20micro/lane. Lane 2-4 transient expression of EZH2

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HCT116

SUZ12 Antibody Western Blot validation (1:1000 dilution) in HCT116 (Cat no:20366-1-AP)