Anticorps Monoclonal anti-SYAP1

SYAP1 Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

2E12A5

N° de cat : 68410-1-Ig

Synonymes

2E12A5, BSD domain-containing signal transducer and Akt interactor protein, BSTA, PRO3113, Synapse associated protein 1

---

Galerie de données de validation

• 

  WB analysis using 68410-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68410-1-Ig (SYAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  IP experiment of L02 using 68410-1-Ig

  IP result of anti-SYAP1 (IP:68410-1-Ig, 4ug; Detection:68410-1-Ig 1:2000) with L02 cells lysate 3080 ug.

• 

  IF Staining of HeLa using 68410-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SYAP1 antibody (68410-1-Ig, Clone: 2E12A5 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

• 

  IF Staining of HepG2 using 68410-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SYAP1 antibody (68410-1-Ig, Clone: 2E12A5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules U2OS, cellules A549, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3
• Résultats positifs en IP: cellules L02,
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68410-1-Ig cible SYAP1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SYAP1 Protéine recombinante Ag9626
• Nom complet: synapse associated protein 1, SAP47 homolog (Drosophila)
• Masse moléculaire calculée: 352 aa, 40 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC014657
• Symbole du gène: SYAP1
• Identification du gène (NCBI): 94056
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SYAP1(Synapse-associated protein 1) is a human homologue of the Drosophila SAP47 (synapse associated protein), which is recognized by a monoclonal antibody that selectively stain synaptic terminals.This protein is a 352 amino acid protein that is ubiquitously expressed in adult tissues. SYAP1 contains one BSD domain which is is an approximately 60 amino acid long protein domain named after the BTF2-like transcription factors, Synapse-associated proteins and DOS2-like proteins in which it is found.

{{ptg:RelatedPrimaryAntibodies}}