Anticorps Polyclonal de lapin anti-SYF2

• Phare
• Validé par KD/KO

SYF2 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, RIP, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (3)

N° de cat : 16958-1-AP

Synonymes

Pre-mRNA-splicing factor SYF2, Pre mRNA splicing factor SYF2, GCIPIP, CCNDBP1-interactor, CCNDBP1 interactor

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Galerie de données de validation

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  WB analysis of HEK-293 using 16958-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16958-1-AP (SYF2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16958-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16958-1-AP (SYF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 16958-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 16958-1-AP (SYF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

16958-1-AP cible SYF2 dans les applications de WB, IF/ICC, RIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SYF2 Protéine recombinante Ag10656
• Nom complet: SYF2 homolog, RNA splicing factor (S. cerevisiae)
• Masse moléculaire calculée: 243 aa, 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC010862
• Symbole du gène: SYF2
• Identification du gène (NCBI): 25949
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,RIP	Nucleic Acids Res; ZRANB2 and SYF2-mediated splicing programs converging on ECT2 are involved in breast cancer cell resistance to doxorubicin.; Authors - Iris Tanaka ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Moritz (Verified Customer) (09-24-2025)

Worked well for Syf2 detection in HEK293T cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T

SYF2 Antibody Western Blot validation (1:3000 dilution) in HEK293T (Cat no:16958-1-AP)

FH

Aleksandr (Verified Customer) (07-19-2025)

A major band on Syf2 is detected. Antibody was incubated overnight at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RKO

SYF2 Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:16958-1-AP)

FH

Tsimafei (Verified Customer) (08-07-2024)

Antibody recognize a strong band of the correct size. Also two lower nolecular weight non-specific bands are visible

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

SYF2 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:16958-1-AP)

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