Anticorps Polyclonal de lapin anti-SYK

• Phare
• Validé par KD/KO

SYK Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 14858-1-AP

Synonymes

spleen tyrosine kinase, SYK, Tyrosine protein kinase SYK

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Galerie de données de validation

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  WB analysis of Raji using 14858-1-AP

  WB result of SYK antibody (14858-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SYK transfected Raji cells.

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  WB analysis using 14858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14858-1-AP (SYK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14858-1-AP (SYK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14858-1-AP (SYK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14858-1-AP (SYK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14858-1-AP (SYK antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14858-1-AP (SYK antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14858-1-AP (SYK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14858-1-AP (SYK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14858-1-AP (SYK antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14858-1-AP (SYK antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14858-1-AP (SYK antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14858-1-AP (SYK antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules Raji, cellules Ramos
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu d'appendicite humain, tissu ovarien humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

14858-1-AP cible SYK dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SYK Protéine recombinante Ag6657
• Nom complet: spleen tyrosine kinase
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 66-72 kDa
• Numéro d’acquisition GenBank: BC001645
• Symbole du gène: SYK
• Identification du gène (NCBI): 6850
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SYK belongs to the protein kinase superfamily, Tyr protein kinase family and SYK/ZAP-70 subfamily. It is positive effector of BCR-stimulated responses. SYK couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway It phosphorylates USP25 and regulates its intracellular levels. SYK also promotes liver fibrosis by activation of hepatic stellate cells and acts as a biomarker for human hepatocellular carcinoma. (PMID: 29537660) SYK can be detected 66-72 kDa in human and mouse (PMID: 37661351).

Publications

Species	Application	Title
mouse	WB	Food Funct; Synergistic effects of L-theanine and epigallocatechin gallate in alleviating ovalbumin allergy by regulating intestinal immunity through inhibition of mast cell degranulation; Authors - Wei Xu
human	IHC	Front Genet; SYK Is Associated With Malignant Phenotype and Immune Checkpoints in Diffuse Glioma.; Authors - Quanwei Zhou
mouse	WB	Animals (Basel); Equus β-Defensin-1 Regulates Innate IMMUNE Response in S. aureus-Infected Mouse Monocyte Macrophage; Authors - Le Pei