Anticorps Polyclonal de lapin anti-SYNJ2BP

• Phare
• Validé par KD/KO

SYNJ2BP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 15666-1-AP

Synonymes

ARIP2, OMP25, Synaptojanin-2-binding protein

---

Galerie de données de validation

• 

  WB analysis using 15666-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15666-1-AP (SYNJ2BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15666-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15666-1-AP (SYNJ2BP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 15666-1-AP

  IP result of anti-SYNJ2BP (IP:15666-1-AP, 3ug; Detection:15666-1-AP 1:500) with mouse lung tissue lysate 4000ug.

• 

  IHC staining of mouse liver using 15666-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15666-1-AP (SYNJ2BP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15666-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15666-1-AP (SYNJ2BP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 15666-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SYNJ2BP antibody (15666-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 15666-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SYNJ2BP antibody (15666-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 15666-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SYNJ2BP (15666-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules A549, tissu pulmonaire de souris, tissu rénal de rat
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules MCF-7
• Résultats positifs en FC (Intra): cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

15666-1-AP cible SYNJ2BP dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SYNJ2BP Protéine recombinante Ag8130
• Nom complet: synaptojanin 2 binding protein
• Masse moléculaire calculée: 145 aa, 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC007704
• Symbole du gène: SYNJ2BP
• Identification du gène (NCBI): 55333
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Activin, a member of the TGF-beta superfamily, plays important roles in reproductive tissues as a stimulator of follicle-stimulating hormone (FSH) secretion and inhibits the proliferation of breast cancer cells. Activin receptor-interacting protein 2 (ARIP2), also known as synaptojanin-2-binding protein (SYNJ2BP), is located in the outer membrane of mitochondria. SYNJ2BP is widely distributed in various human tissues, and has been recently identified in mouse tissues as a regulatory protein of activin signal transduction by interacting with activin type II receptors. SYNJ2BP may be a putative growth-promoting factor involved in breast tumorigenesis and tumor development. A double band of size 15.9 kDa can be detected in Western blot (PMID: 23284606).

Publications

Species	Application	Title
human	WB	Science; The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.; Authors - Michael J McKenna
	IF	Neuron; Neuronal mitochondria transport Pink1 mRNA via synaptojanin 2 to support local mitophagy.; Authors - Angelika B Harbauer
	WB	Sci Adv; ARMC1 partitions between distinct complexes and assembles MIRO with MTFR to control mitochondrial distribution; Authors - Michael J McKenna
human	WB	Cell Rep; Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.; Authors - Fenglei Jian
mouse,human	WB,IP,IHC	Oncotarget; SYNJ2BP promotes the degradation of PTEN through the lysosome-pathway and enhances breast tumor metastasis via PI3K/AKT/SNAI1 signaling.; Authors - Miao Wang ; KD Validated
human	WB	J Cell Sci; Mitochondrial antiviral-signalling protein is a client of the BAG6 protein quality control complex.; Authors - Peristera Roboti

{{ptg:RelatedPrimaryAntibodies}}