Anticorps Polyclonal de lapin anti-SYNM

SYNM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 20735-1-AP

Synonymes

Synemin, DMN, Desmuslin

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Galerie de données de validation

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  WB analysis using 20735-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20735-1-AP (SYNM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. SYNM gene methylation in T-47D cells causes gene silencing.

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  WB analysis of human skeletal muscle using 20735-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20735-1-AP (SYNM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 20735-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 20735-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 20735-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 20735-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human bladder using 20735-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human bladder using 20735-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 20735-1-AP

  Immunofluorescent analysis of HeLa cells using 20735-1-AP (SYNM antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules Y79, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu cardiaque humain, tissu vésical humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

20735-1-AP cible SYNM dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: synemin, intermediate filament protein
• Masse moléculaire calculée: 173 kDa
• Poids moléculaire observé: 170-180 kDa
• Numéro d’acquisition GenBank: NM_015286
• Symbole du gène: SYNM
• Identification du gène (NCBI): 23336
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SYNM, also named as DMN, KIAA0353 and SYN, belongs to the intermediate filament family. SYNM is a Type-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteropolymeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteropolymeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells. The antibody can't recognize isoform 2 or 3 of SYNM.

Publications

Species	Application	Title
human	IHC	J Ovarian Res; A risk model of gene signatures for predicting platinum response and survival in ovarian cancer.; Authors - Siyu Chen
human	IF	bioRxiv; Tropism of SARS-CoV-2 for Developing Human Cortical Astrocytes.; Authors - Madeline G Andrews

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