Anticorps Polyclonal de lapin anti-SYNPO

SYNPO Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(70)

N° de cat : 21064-1-AP

Synonymes

Synaptopodin, snaptopodin, KIAA1029

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Galerie de données de validation

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  WB analysis of mouse brain using 21064-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21064-1-AP (Synaptopodin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 21064-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21064-1-AP (Synaptopodin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 21064-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21064-1-AP (Synaptopodin antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21064-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21064-1-AP (Synaptopodin antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of rat brain using 21064-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Synaptopodin antibody (21064-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse kidney using 21064-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SYNPO antibody (21064-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de rat, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 33 publications below
• IHC: See 8 publications below
• IF: See 42 publications below

Informations sur le produit

21064-1-AP cible SYNPO dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SYNPO Protéine recombinante Ag15089
• Nom complet: synaptopodin
• Masse moléculaire calculée: 929 aa, 99 kDa
• Poids moléculaire observé: 99-115 kDa
• Numéro d’acquisition GenBank: BC146665
• Symbole du gène: SYNPO
• Identification du gène (NCBI): 11346
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

Publications

Species	Application	Title
mouse	IF	J Extracell Vesicles; Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease; Authors - Rong Yang
human	IF	Biofabrication; A glomerulus chip with spherically twisted cell-laden hollow fibers as glomerular capillary tufts; Authors - Zhilin Dai
human	IF	Theranostics; Renal tubular Bim mediates the tubule-podocyte crosstalk via NFAT2 to induce podocyte cytoskeletal dysfunction.; Authors - Chunmei Xu
human,mouse	IF	Diabetes; Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.; Authors - Hua Qu
mouse	IF	Free Radic Biol Med; P2X7R/AKT/mTOR signaling mediates high glucose-induced decrease in podocyte autophagy; Authors - Cheng Qian
mouse	WB,IF	Mol Ther Nucleic Acids; Inhibition of the lncRNA MIAT prevents podocyte injury and mitotic catastrophe in diabetic nephropathy.; Authors - Ziyang Wang

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