Anticorps Monoclonal anti-SYNPO

SYNPO Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

1E1B1

N° de cat : 67339-1-Ig

Synonymes

Synaptopodin, 1E1B1, KIAA1029, snaptopodin

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Galerie de données de validation

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  WB analysis of rat brain using 67339-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67339-1-Ig (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67339-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67339-1-Ig (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67339-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67339-1-Ig (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 67339-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67339-1-Ig (SYNPO antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67339-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67339-1-Ig (SYNPO antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 67339-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67339-1-Ig (SYNPO antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 67339-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SYNPO antibody (67339-1-Ig, Clone: 1E1B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse kidney using 67339-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SYNPO antibody (67339-1-Ig, Clone: 1E1B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 67339-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SYNPO antibody (67339-1-Ig, Clone: 1E1B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu cérébral de porc, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de rat, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris, tissu rénal de souris
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:750-1:3000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

67339-1-Ig cible SYNPO dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SYNPO Protéine recombinante Ag15089
• Nom complet: synaptopodin
• Masse moléculaire calculée: 929 aa, 99 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC146665
• Symbole du gène: SYNPO
• Identification du gène (NCBI): 11346
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

Publications

Species	Application	Title
mouse	WB	Front Cell Dev Biol; Umbilical Cord Mesenchymal Stem Cells Ameliorate Kidney Injury in MRL/Ipr Mice Through the TGF-β1 Pathway.; Authors - Chunkai Huang
mouse	WB	Cell Death Dis; Sestrin2 remedies podocyte injury via orchestrating TSP-1/TGF-β1/Smad3 axis in diabetic kidney disease.; Authors - Shan Song
mouse	IF	Inflammation; Canagliflozin Attenuates Podocyte Inflammatory Injury through Suppressing the TXNIP/NLRP3 Signaling Pathway in Diabetic Kidney Disease Mice; Authors - Siyu Li

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