Anticorps Polyclonal de lapin anti-Synaptoporin

Synaptoporin Polyclonal Antibody for WB, IHC, IF-Fro, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-Fro, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 14143-1-AP

Synonymes

SYNPR, SPO

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Galerie de données de validation

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  WB analysis using 14143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14143-1-AP (Synaptoporin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14143-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14143-1-AP (Synaptoporin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 14143-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14143-1-AP (Synaptoporin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14143-1-AP

  IP result of anti-Synaptoporin (IP:14143-1-AP, 4ug; Detection:14143-1-AP 1:2000) with mouse brain tissue lysate 1720 ug.

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  IHC staining of mouse brain using 14143-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14143-1-AP (Synaptoporin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14143-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14143-1-AP (Synaptoporin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14143-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14143-1-AP (Synaptoporin antibody at dilution of 1:50.

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  IHC staining of human gliomas using 14143-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14143-1-AP (Synaptoporin antibody at dilution of 1:50.

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  IHC staining of human colon using 14143-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14143-1-AP (Synaptoporin antibody at dilution of 1:50.

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  IHC staining of human colon using 14143-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14143-1-AP (Synaptoporin antibody at dilution of 1:50.

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  IHC staining of human brain using 14143-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14143-1-AP (Synaptoporin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 14143-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14143-1-AP (Synaptoporin antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of rat brain using 14143-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using Synaptoporin antibody (14143-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain, tissu de cervelet de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain, tissu de côlon humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-Fro: tissu cérébral de rat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below

Informations sur le produit

14143-1-AP cible Synaptoporin dans les applications de WB, IHC, IF-Fro, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Synaptoporin Protéine recombinante Ag5307
• Nom complet: synaptoporin
• Masse moléculaire calculée: 265 aa, 29 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC022518
• Symbole du gène: Synaptoporin
• Identification du gène (NCBI): 132204
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Biomedicines; Combined DiI and Antibody Labeling Reveals Complex Dysgenesis of Hippocampal Dendritic Spines in a Mouse Model of Fragile X Syndrome; Authors - Luisa Speranza
mouse	IF	CNS Neurosci Ther; Inhibition of ANXA2 activity attenuates epileptic susceptibility and GluA1 phosphorylation; Authors - Limin Ma

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Cajsa (Verified Customer) (10-07-2024)

Great antibody!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Myenteric plexus of adult mouse intestine

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