Anticorps Polyclonal de lapin anti-Synaptotagmin-12

Synaptotagmin-12 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 55015-1-AP

Synonymes

SRG1, Synaptotagmin 12, synaptotagmin XII, Synaptotagmin-12, SYT12, SytXII

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Galerie de données de validation

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  WB analysis of mouse brain using 55015-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55015-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 55015-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 55015-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

55015-1-AP cible Synaptotagmin-12 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: synaptotagmin XII
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: NM_177963
• Symbole du gène: Synaptotagmin-12
• Identification du gène (NCBI): 91683
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SYT12 belongs to the synaptotagmin family. It may be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis. The antibody is specific to STY12.

Publications

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; Proteomic Profile of Mouse Brain Aging Contributions to Mitochondrial Dysfunction, DNA Oxidative Damage, Loss of Neurotrophic Factor, and Synaptic and Ribosomal Proteins.; Authors - Yingchao Li
mouse	WB,IF	Nat Neurosci; Adaptor protein AP-3 produces synaptic vesicles that release at high frequency by recruiting phospholipid flippase ATP8A1; Authors - Hongfei Xu
human	WB	BMC Cancer; Integration of bioinformatics and cellular experiments unveils the role of SYT12 in gastric cancer; Authors - Xingdong Niu

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