Anticorps Polyclonal de lapin anti-Siglec-15

Siglec-15 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 28594-1-AP

Synonymes

CD33 antigen like 3, CD33L3, HsT1361, SIGLEC 15, SIGLEC15

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Galerie de données de validation

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  WB analysis of A549 using 28594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28594-1-AP (Siglec-15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules NCI-H1299, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

28594-1-AP cible Siglec-15 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Siglec-15 Protéine recombinante Ag29829
• Nom complet: sialic acid binding Ig-like lectin 15
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 38-40 kDa
• Numéro d’acquisition GenBank: NM_213602
• Symbole du gène: Siglec-15
• Identification du gène (NCBI): 284266
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sialic acid binding Ig-like lectin 15 (Siglec-15) is a member of the Siglec family of glycan-recognition proteins. Siglec-15 is a type-I transmembrane protein consisting of two immunoglobulin (Ig)-like domains, a transmembrane domain, and a short cytoplasmic tail (PMID: 17483134). It is mainly expressed on a subset of myeloid lineage cells and can be upregulated in many cancers (PMID: 32939323). Siglec-15 has been reported as a immune suppressor and a potential target for normalization cancer immunotherapy (PMID: 30833750). Siglec-15 has a calculated molecular weight of 36 kDa. It has been detected at 38-40 kDa due to glycosylation (PMID: 32921411).

Publications

Species	Application	Title
human,mouse,pig	WB	Cell Mol Life Sci; Siglec-15 is a putative receptor for porcine epidemic diarrhea virus infection; Authors - Zhihua Feng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rebecca (Verified Customer) (07-21-2022)

Positive staining identified at 1:500 dilution in patient FFPE tissue. Slight background.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Prostate cancer FFPE tissue

Siglec-15 Antibody Immunofluorescence validation (1:500 dilution) in Prostate cancer FFPE tissue (Cat no:28594-1-AP)

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