Anticorps Recombinant de lapin anti-Stathmin 1

• Phare
• Validé par KD/KO

Stathmin 1 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

2H6

Avis (1)

N° de cat : 82559-1-RR

Synonymes

STMN1, 2H6, C1orf215, LAP18, Leukemia-associated phosphoprotein p18

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Galerie de données de validation

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  WB analysis using 82559-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82559-1-RR (Stathmin 1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 82559-1-RR

  WB result of Stathmin 1 antibody (82559-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Stathmin 1 transfected HepG2 cells.

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  IHC staining of rat brain using 82559-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82559-1-RR (Stathmin 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 82559-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82559-1-RR (Stathmin 1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 82559-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82559-1-RR (Stathmin 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 82559-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Stathmin 1 antibody (82559-1-RR, Clone: 2H6 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 82559-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Stathmin 1 antibody (82559-1-RR, Clone: 2H6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 82559-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using Stathmin 1 antibody (82559-1-RR, Clone: 2H6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 82559-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82559-1-RR against Human Stathmin 1 were performed. The affinity constant is 7.57 nM.

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules HepG2, cellules Jurkat, cellules PC-12
• Résultats positifs en IHC: tissu cérébral de rat, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules HeLa, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82559-1-RR cible Stathmin 1 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Stathmin 1 Protéine recombinante Ag1633
• Nom complet: stathmin 1/oncoprotein 18
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC014353
• Symbole du gène: Stathmin 1
• Identification du gène (NCBI): 3925
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Frédéric (Verified Customer) (11-22-2024)

Good detection of stathmin-1 protein at expected molecular weight.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: A431cells - P12 cells - rat pituitary cells

Stathmin 1 Antibody Western Blot validation (1/1000 dilution) in A431cells - P12 cells - rat pituitary cells (Cat no:82559-1-RR)

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