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Anticorps Polyclonal de lapin anti-Synaptophysin

Synaptophysin Polyclonal Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

N° de cat : CL647-17785

Synonymes

synaptophysin, SYP

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using CoraLite® Plus 647-conjugated Synaptophysin Polycl (CL647-17785)

    IF Staining of mouse pancreas using CL647-17785

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-17785) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using CoraLite® Plus 647-conjugated Synaptophysin Polycl (CL647-17785)

    IF Staining of mouse pancreas using CL647-17785

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-17785) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 647-conjugated Synaptophysin Polycl (CL647-17785)

    IF Staining of mouse brain using CL647-17785

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-17785) at dilution of 1:1000.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 647-conjugated Synaptophysin Polycl (CL647-17785)

    IF Staining of mouse brain using CL647-17785

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-17785) at dilution of 1:1000.

  • Flow cytometry (FC) experiment of PC-12 cells using CoraLite® Plus 647-conjugated Synaptophysin Polycl (CL647-17785)

    FC experiment of PC-12 using CL647-17785

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Synaptophysin (CL647-17785) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFtissu pancréatique de souris, tissu cérébral de souris
Résultats positifs en cytométriecellules PC-12

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-17785 cible Synaptophysin dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Synaptophysin Protéine recombinante Ag11781
Nom complet synaptophysin
Masse moléculaire calculée 313 aa, 34 kDa
Poids moléculaire observé 38-40 kDa
Numéro d’acquisition GenBankBC064550
Symbole du gène SYP
Identification du gène (NCBI) 6855
Conjugaison CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Protocole

Product Specific Protocols
IF protocol for CL Plus 647 Synaptophysin antibody CL647-17785Download protocol
Standard Protocols
Click here to view our Standard Protocols