Anticorps Polyclonal de lapin anti-transgelin/SM22

• Phare
• Validé par KD/KO

transgelin/SM22 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF-P, IF-Fro, IP, ELISA

Conjugaison

Non conjugué

Publications(156)

Avis (1)

N° de cat : 10493-1-AP

Synonymes

SM22, transgelin, 22 kDa actin-binding protein, Protein WS3-10, SM22-a

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Galerie de données de validation

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  WB analysis of mouse colon using 10493-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10493-1-AP (transgelin/SM22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10493-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10493-1-AP (transgelin/SM22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 10493-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 10493-1-AP (transgelin/SM22 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 10493-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 10493-1-AP (transgelin/SM22 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 10493-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10493-1-AP (transgelin/SM22 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10493-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10493-1-AP (transgelin/SM22 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 10493-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10493-1-AP (transgelin/SM22 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 10493-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10493-1-AP (transgelin/SM22 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 10493-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10493-1-AP (transgelin/SM22 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10493-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10493-1-AP (transgelin/SM22 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10493-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10493-1-AP (transgelin/SM22 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 10493-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 10493-1-AP (transgelin/SM22 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 10493-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 10493-1-AP (transgelin/SM22 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 10493-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using transgelin/SM22 antibody (10493-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 10493-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 10493-1-AP (transgelin/SM22 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 10493-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 10493-1-AP (transgelin/SM22 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 10493-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse heart tissue using 10493-1-AP (transgelin/SM22 antibody) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de côlon de souris, cellules Caco-2, tissu de côlon de rat, tissu de côlon humain, tissu d'estomac de souris
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque humain, tissu cérébral de souris, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris,
• Résultats positifs en IF-Fro: tissu cardiaque de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 110 publications below
• IHC: See 22 publications below
• IF: See 49 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

10493-1-AP cible transgelin/SM22 dans les applications de WB, IHC, IF-P, IF-Fro, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: transgelin/SM22 Protéine recombinante Ag0764
• Nom complet: transgelin
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 19-22 kDa
• Numéro d’acquisition GenBank: BC004927
• Symbole du gène: transgelin/SM22
• Identification du gène (NCBI): 6876
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The transgelin family is a protein group belonging to the 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, or SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full-length transgelin 1 protein. It can cross-react with other two transgenes based on the sequence similarity.

Publications

Species	Application	Title
mouse	IF	Cell; Mapping the Fine-Scale Organization and Plasticity of the Brain Vasculature.; Authors - Christoph Kirst
mouse	WB,IF	Cell Host Microbe; Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation; Authors - Zhenyu Tian
mouse	IF	Acta Pharm Sin B; The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.; Authors - Weimin Yu
mouse	IHC	J Exp Med; Dependence of proliferative vascular smooth muscle cells on CD98hc (4F2hc, SLC3A2).; Authors - Fogelstrand Per P
mouse	IF	Nat Commun; Hyperlipidemia-induced cholesterol crystal production by endothelial cells promotes atherogenesis.; Authors - Yvonne Baumer
mouse	IF	Circ Res; Peptide-mediated disruption of calmodulin-cyclin E interactions inhibits proliferation of vascular smooth muscle cells and neointima formation.; Authors - Hui Sonya S

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ying (Verified Customer) (08-19-2019)

very good antibody

• Applications: Western Blot,
• Primary Antibody Dilution: 1000
• Cell Tissue Type: primary mouse cardiomyocytes

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