Anticorps Monoclonal anti-transgelin/SM22

transgelin/SM22 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(19)

CloneNo.

2A10C2

Avis (1)

N° de cat : 60213-1-Ig

Synonymes

SM22, transgelin, 22 kDa actin-binding protein, 2A10C2, Protein WS3-10

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Galerie de données de validation

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  WB analysis using 60213-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 60213-1-Ig

  human colon tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60213-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig stomach using 60213-1-Ig

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 60213-1-Ig

  rat colon tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat stomach using 60213-1-Ig

  rat stomach tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 60213-1-Ig

  human stomach tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 60213-1-Ig

  human colon tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 60213-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 60213-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 60213-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 60213-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon using 60213-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 60213-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse heart using 60213-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using transgelin/SM22 antibody (60213-1-Ig, Clone: 2A10C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 60213-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse heart using 60213-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de côlon de porc, tissu de côlon de rat, tissu de côlon de souris, tissu de côlon humain, tissu d'estomac de lapin, tissu d'estomac de porc, tissu d'estomac de rat, tissu d'estomac humain
• Résultats positifs en IHC: tissu de côlon humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:400
• Immunofluorescence (IF)-P: IF-P : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IF: See 11 publications below

Informations sur le produit

60213-1-Ig cible transgelin/SM22 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: transgelin/SM22 Protéine recombinante Ag0764
• Nom complet: transgelin
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 18+22 kDa
• Numéro d’acquisition GenBank: BC004927
• Symbole du gène: transgelin/SM22
• Identification du gène (NCBI): 6876
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.

Publications

Species	Application	Title
human	WB,IF	Dev Cell; Endothelial progenitor cells control remodeling of uterine spiral arteries for the establishment of utero-placental circulation; Authors - Bin Tan
human	IF	Pharmacol Res; LDHA mediated degradation of extracellular matrix is a potential target for the treatment of aortic dissection.; Authors - Xiaohui Wu
mouse	WB	Front Cell Dev Biol; S-propargyl-cysteine promotes the stability of atherosclerotic plaque via maintaining vascular muscle contractile phenotype; Authors - Li Ping
human	WB	Oncotarget; Specific role of RhoC in tumor invasion and metastasis.; Authors - Sarah Lang
mouse	WB	Front Cell Dev Biol; Platelet-Derived Microvesicles Promote VSMC Dedifferentiation After Intimal Injury via Src/Lamtor1/mTORC1 Signaling; Authors - Ji-Ting Liu
rat	IF	Biochim Biophys Acta Mol Basis Dis; Methamphetamine induces thoracic aortic aneurysm/dissection through C/EBPβ.; Authors - Bao-Ying Luo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ana Luísa (Verified Customer) (07-25-2022)

No permeabilization. Blocking with 5%FBS and 2%BSA. Some background

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Intervertebral disc cells

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