Anticorps Polyclonal de lapin anti-Transgelin 2

• Phare
• Validé par KD/KO

Transgelin 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(23)

Avis (1)

N° de cat : 10234-2-AP

Synonymes

TAGLN2, Transgelin-2, Transgelin 2/TAGLN2, SM22-alpha homolog, Epididymis tissue protein Li 7e

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Galerie de données de validation

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  WB analysis using 10234-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10234-2-AP (Transgelin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 10234-2-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 10234-2-AP (Transgelin 2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10234-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10234-2-AP (Transgelin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10234-2-AP (Transgelin 2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10234-2-AP (Transgelin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10234-2-AP (Transgelin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10234-2-AP (Transgelin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10234-2-AP (Transgelin 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10234-2-AP (Transgelin 2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human urothelial carcinoma using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10234-2-AP (Transgelin 2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10234-2-AP (Transgelin 2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human urothelial carcinoma using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10234-2-AP (Transgelin 2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human urothelial carcinoma using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10234-2-AP (Transgelin 2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10234-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Transgelin 2 antibody (10234-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 10234-2-AP

  Immunofluorescent analysis of HepG2 cells using 10234-2-AP (Transgelin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293
• Résultats positifs en IHC: tissu de cancer de la peau humain, tissu de cancer du côlon humain, tissu de carcinome urothélial humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 18 publications below
• IHC: See 9 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below

Informations sur le produit

10234-2-AP cible Transgelin 2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Transgelin 2 Protéine recombinante Ag0337
• Nom complet: transgelin 2
• Masse moléculaire calculée: 199 aa, 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC002616
• Symbole du gène: Transgelin-2/TAGLN2
• Identification du gène (NCBI): 8407
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgenlin 2 protein. It may cross react with other two transgelins based on the sequence similarity.

Publications

Species	Application	Title
human	ELISA	Mol Cell Proteomics; Comparative Tissue Proteomics of Microdissected Specimens Reveals Novel Candidate Biomarkers of Bladder Cancer.; Authors - Chien-Lun Chen
mouse	WB	J Am Heart Assoc; Multi-Omic Insight Into the Molecular Networks in the Pathogenesis of Coronary Artery Disease; Authors - Qinghua Fang
human	WB,IHC	J Proteome Res; Identification of vascular breast tumor markers by laser capture microdissection and label-free LC-MS.; Authors - Hill Jennifer J JJ
human	WB,IHC	J Proteome Res; Tissue proteomics reveals differential and compartment-specific expression of the homologs transgelin and transgelin-2 in lung adenocarcinoma and its stroma.; Authors - Rho Jung-Hyun JH
human	IHC	J Proteome Res; Post-translational modifications and protein-specific isoforms in endometriosis revealed by 2D DIGE.; Authors - Stephens Andrew N AN
human	WB	Front Oncol; Prognosis Analysis and Validation of m6A Signature and Tumor Immune Microenvironment in Glioma.; Authors - Shaojian Lin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (06-16-2019)

It recognizes transgelin (prominent lower band) and some other bands, albeit much more weakly.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

Transgelin 2 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10234-2-AP)

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