Anticorps Monoclonal anti-Transgelin 2

• Phare
• Validé par KD/KO

Transgelin 2 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

2E5G1

N° de cat : 60044-1-Ig

Synonymes

SM22 alpha homolog, SM22 beta, SM22beta, TAGLN2, Transgelin 2, Transgelin 2/TAGLN2

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Galerie de données de validation

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  WB analysis of K-562 using 60044-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 60044-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 60044-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 60044-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 60044-1-Ig

  IP Result of anti-Transgelin 2 (IP:60044-1-Ig, 4ug; Detection:60044-1-Ig 1:1000) with HEK-293 cells lysate 1000ug.

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  IHC staining of human urothelial carcinoma using 60044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 60044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 60044-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 60044-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 60044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 60044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 60044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 60044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 60044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human colon cancer using 60044-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Transgelin 2 antibody (60044-1-Ig, Clone: 2E5G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human colon cancer using 60044-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Transgelin 2 antibody (60044-1-Ig, Clone: 2E5G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 60044-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Transgelin 2 antibody (60044-1-Ig, Clone: 2E5G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules K562, cellules K-562
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de carcinome urothélial humain, tissu de cancer du côlon humain, tissu de côlon de souris, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du côlon humain, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

60044-1-Ig cible Transgelin 2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Transgelin 2 Protéine recombinante Ag0337
• Nom complet: transgelin 2
• Masse moléculaire calculée: 199 aa, 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC002616
• Symbole du gène: TAGLN2
• Identification du gène (NCBI): 8407
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. Our test result showed that this antibody Is specific to TAGLN2. It does not bind TAGLN1 and TAGLN3.

Publications

Species	Application	Title
human	WB	Mol Med Rep; Downregulation of transgelin 2 promotes breast cancer metastasis by activating the reactive oxygen species/nuclear factor‑κB signaling pathway.; Authors - Liu Yang ; KD Validated
human,mouse	WB,IF	Nat Commun; Soluble TREM2 ameliorates tau phosphorylation and cognitive deficits through activating transgelin-2 in Alzheimer's disease; Authors - Xingyu Zhang