Anticorps Polyclonal de lapin anti-TAL1

TAL1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 55317-1-AP

Synonymes

bHLHa17, SCL, Stem cell protein, tal 1, TAL1, TCL5

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Galerie de données de validation

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  WB analysis of Raji using 55317-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 55317-1-AP (TAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 55317-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 55317-1-AP (TAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 55317-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55317-1-AP (TAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 55317-1-AP

  IP result of anti-TAL1 (IP:55317-1-AP, 4ug; Detection:55317-1-AP 1:1000) with Raji cells lysate 2000ug.

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules Jurkat
• Résultats positifs en IP: cellules Raji

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

55317-1-AP cible TAL1 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, poisson-zèbre
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: T-cell acute lymphocytic leukemia 1
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 40-44 kDa
• Numéro d’acquisition GenBank: NM_003189
• Symbole du gène: TAL1
• Identification du gène (NCBI): 6886
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

T-cell acute lymphocytic leukemia protein 1 (TAL1) also named Stem cell protein,T-cell leukemia/lymphoma protein 5.It is specifically expressed in leukemic stem cell,implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation, serving as a positive regulator of erythroid differentiation. The 38kd, 44kd bands recognized by antibody are the phosphorylated forms of TAL1 and it's isoforms.

Publications

Species	Application	Title
human	WB	Pharmaceuticals (Basel); Caulis Polygoni Multiflori Accelerates Megakaryopoiesis and Thrombopoiesis via Activating PI3K/Akt and MEK/ERK Signaling Pathways; Authors - Xin Yang
zebrafish	WB	Development; BF170 hydrochloride enhances the emergence of hematopoietic stem and progenitor cells; Authors - WenYe Liu
		Nat Immunol; Tumor cell-released kynurenine biases MEP differentiation into megakaryocytes in individuals with cancer by activating AhR-RUNX1; Authors - Li Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wojciech (Verified Customer) (12-19-2022)

TAl1 polyclonal worked well with transfected lysate by WB at 1:1000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Jurkat and Karpas45 cells

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