Anticorps Polyclonal de lapin anti-TAOK1

TAOK1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 26250-1-AP

Synonymes

hKFC B, KIAA1361, Kinase from chicken homolog B, MAP3K16, MARKK, PSK2, STE20 like kinase PSK2, TAO kinase 1, TAO1, TAOK1

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Galerie de données de validation

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  WB analysis of HeLa using 26250-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26250-1-AP (TAOK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 26250-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26250-1-AP (TAOK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 26250-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26250-1-AP (TAOK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 26250-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26250-1-AP (TAOK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 26250-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26250-1-AP (TAOK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu testiculaire de souris
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:100-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 5 publications below

Informations sur le produit

26250-1-AP cible TAOK1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TAOK1 Protéine recombinante Ag22461
• Nom complet: TAO kinase 1
• Masse moléculaire calculée: 1001 aa, 116 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: BC133039
• Symbole du gène: TAOK1
• Identification du gène (NCBI): 57551
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Thousand and one kinase 1 (TAOK1), also known as prostate-derived sterile 20 (Ste20)-like kinase 2, TAO1 (thousand and one amino-acid protein 1) or MARKK, is a member of the MAP3K protein kinase and belongs to the germinal-center kinase-like class of sterile 20 (Ste20)-like kinases (PMID: 29400705). TAOK1 is involved in mammalian brain development and functions as a negative regulator in IL-17-mediated signaling and inflammation (PMID: 33565190). TAOK1 has a calculated molecular mass of 116 kDa and encodes a serine/threonine protein kinase at its N terminus.

Publications

Species	Application	Title
human	WB,IF	Am J Hum Genet; Variants in EXOSC9 Disrupt the RNA Exosome and Result in Cerebellar Atrophy with Spinal Motor Neuronopathy.; Authors - David T Burns
human	WB,IF	Acta Neuropathol Commun; A new TAO kinase inhibitor reduces tau phosphorylation at sites associated with neurodegeneration in human tauopathies.; Authors - Caterina Giacomini
mouse	IF	Sci Signal; Neurodevelopmental disorder-associated mutations in TAOK1 reveal its function as a plasma membrane remodeling kinase; Authors - Neal Beeman
human	WB,IF	Mol Cancer Ther; Targeting TAO kinases using a new inhibitor compound delays mitosis and induces mitotic cell death in centrosome amplified breast cancer cells.; Authors - Chuay-Yeng Koo
monkey	WB	J Cell Sci; Rnd3 interacts with TAO kinases and contributes to mitotic cell rounding and spindle positioning.; Authors - Ritu Garg
human	WB,IF	Nat Commun; Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA; Authors - Mirjana Malnar Črnigoj