Anticorps Monoclonal anti-TAPBPL

TAPBPL Monoclonal Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1D12C2

N° de cat : 68577-1-Ig

Synonymes

TAP-binding protein-like, Tapasin-related protein, TAPASIN-R, Tapasin-like, TAPASIN R

---

Galerie de données de validation

• 

  WB analysis using 68577-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68577-1-Ig (TAPBPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 68577-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68577-1-Ig (TAPBPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of A431 using 68577-1-Ig

  1x10^6 A431 cells were intracellularly stained with 0.4 ug TAPBPL Monoclonal antibody (68577-1-Ig, Clone: 1D12C2) and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002) (red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: SCaBER cells, cellules A431, cellules Jurkat, cellules MCF-7, cellules MG U-87, tissu placentaire humain
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68577-1-Ig cible TAPBPL dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TAPBPL Protéine recombinante Ag14371
• Nom complet: TAP binding protein-like
• Masse moléculaire calculée: 468 aa, 50 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC015017
• Symbole du gène: TAPBPL
• Identification du gène (NCBI): 55080
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TAPBPL, also named as TAPBP-R and TAPASIN-R, is a member of the variable-constant Ig superfamily that links MHC class I molecules to the transporter associated with antigen processing in the endoplasmic reticulum.

{{ptg:RelatedPrimaryAntibodies}}