Anticorps Polyclonal de lapin anti-TBC1D1

• Phare
• Validé par KD/KO

TBC1D1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(12)

Avis (1)

N° de cat : 22124-1-AP

Synonymes

KIAA1108, TBC, TBC1, TBC1 domain family member 1, TBC1D1

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Galerie de données de validation

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  WB analysis of LNCaP using 22124-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22124-1-AP (TBC1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 22124-1-AP

  WB result of TBC1D1 antibody (22124-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBC1D1 transfected HEK-293T cells.

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  WB analysis using 22124-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22124-1-AP (TBC1D1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22124-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22124-1-AP (TBC1D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 22124-1-AP

  IP result of anti-TBC1D1 (IP:22124-1-AP, 4ug; Detection:22124-1-AP 1:1000) with Jurkat cells lysate 2400ug.

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  IHC staining of human testis using 22124-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22124-1-AP (TBC1D1 Antibody) at dilution of 1:50.

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  IHC staining of human testis using 22124-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22124-1-AP (TBC1D1 Antibody) at dilution of 1:50.

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  IHC staining of mouse kidney using 22124-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22124-1-AP (TBC1D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 22124-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22124-1-AP (TBC1D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 22124-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22124-1-AP (TBC1D1 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 22124-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22124-1-AP (TBC1D1 Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293T, cellules HeLa, cellules Jurkat, cellules MCF-7
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu rénal de souris, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

22124-1-AP cible TBC1D1 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TBC1D1 Protéine recombinante Ag5406
• Nom complet: TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
• Masse moléculaire calculée: 133 kDa
• Poids moléculaire observé: 150-165 kDa
• Numéro d’acquisition GenBank: BC050321
• Symbole du gène: TBC1D1
• Identification du gène (NCBI): 23216
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Metab; Skeletal muscle from TBC1D4 p.Arg684Ter variant carriers is severely insulin resistant but exhibits normal metabolic responses during exercise; Authors - Jonas M Kristensen
human	WB,IP	Diabetes; Illumination of the Endogenous Insulin-Regulated TBC1D4 Interactome in Human Skeletal Muscle.; Authors - Jeppe K Larsen
mouse	WB	Mol Nutr Food Res; Quercetin oppositely regulates insulin-mediated glucose disposal in skeletal muscle under normal and inflammatory conditions: The dual roles of AMPK activation.; Authors - Kang Liu
human	WB	J Physiol; The insulin-sensitizing effect of a single exercise bout is similar in type I and type II human muscle fibres.; Authors - Magnus R Larsen
		J Biol Chem; Cooperative actions of Tbc1d1 and AS160/Tbc1d4 in GLUT4 trafficking activities.; Authors - Hiroyasu Hatakeyama
mouse	WB	J Pharmacol Sci; PMID: 30733181; Authors - Zejun Mo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Q (Verified Customer) (06-01-2021)

Excellent to detect TBC1D1 in postmortem human samples with WB with the right MW.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: postmortem human brain lysate

TBC1D1 Antibody Western Blot validation (1:2000 dilution) in postmortem human brain lysate (Cat no:22124-1-AP)

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