Anticorps Polyclonal de lapin anti-TBC1D22A

TBC1D22A Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 18332-1-AP

Synonymes

C22orf4, HSC79E021, TBC1 domain family member 22A, TBC1 domain family, member 22A, TBC1D22A

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Galerie de données de validation

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  WB analysis of HeLa using 18332-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18332-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 18332-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 18332-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18332-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 18332-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 18332-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 18332-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 18332-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervix using 18332-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 18332-1-AP (TBC1D22A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervix using 18332-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 18332-1-AP (TBC1D22A antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 18332-1-AP

  Immunofluorescent analysis of HeLa cells using 18332-1-AP (TBC1D22A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COLO 320
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de col de l'utérus humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

18332-1-AP cible TBC1D22A dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TBC1D22A Protéine recombinante Ag12942
• Nom complet: TBC1 domain family, member 22A
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC001292
• Symbole du gène: TBC1D22A
• Identification du gène (NCBI): 25771
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.