Anticorps Polyclonal de lapin anti-TBP

TBP Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

66

Avis (1)

N° de cat : 22006-1-AP

Synonymes

GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis of COLO 320 using 22006-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22006-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 22006-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22006-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 22006-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22006-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 22006-1-AP

  IP Result of anti-TBP (IP:22006-1-AP, 4ug; Detection:22006-1-AP 1:500) with COLO 320 cells lysate 400ug.

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  IHC staining of human breast cancer using 22006-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22006-1-AP (TBP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of mouse testis using 22006-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22006-1-AP (TBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 22006-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 22006-1-AP (TBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22006-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22006-1-AP (TBP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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Applications testées

• Résultats positifs en WB: cellules COLO 320
• Résultats positifs en IP: cellules COLO 320
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu hépatique de rat, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 60 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

22006-1-AP cible TBP dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TBP Protéine recombinante Ag12383
• Nom complet: TATA box binding protein
• Masse moléculaire calculée: 338 aa, 38 kDa
• Poids moléculaire observé: mouse/rat 33-36 kDa and human 37-43kDa
• Numéro d’acquisition GenBank: BC110341
• Symbole du gène: TBP
• Identification du gène (NCBI): 6908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.

Publications

Species	Application	Title
mouse	WB	Hepatology; Steatotic hepatocytes release mature VLDL via methionine and tyrosine metabolism in a Keap1-Nrf2 dependent manner.; Authors - Akitoshi Sano
human	WB	Nat Commun; Menin enhances c-Myc-mediated transcription to promote cancer progression.; Authors - Gongwei Wu
human	WB	Elife; KPNB1 mediates PER/CRY nuclear translocation and circadian clock function.; Authors - Yool Lee
human	WB	J Hazard Mater; ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes.; Authors - Xuan Lai
human	WB	Cell Death Dis; 4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer; Authors - Yanan Wang
human	WB	EMBO Rep; LncRNA GUARDIN suppresses cellular senescence through a LRP130-PGC1α-FOXO4-p21-dependent signaling axis.; Authors - Xuedan Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mi (Verified Customer) (06-25-2023)

This antibody works very well in human white and brown adipocyte cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: human adipocytes

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